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Does proteasome regulate the level of microRNA-1 in cardiomyocytes? Application to anoxia-reoxygenation

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Abstract

Proteasome and microRNAs play a critical role in almost all processes in a living organism, including pathology of the heart; however, their interaction is still in question. In the present study, we have found that proteasome inhibitor provoked increase of mature but not immature microRNA-1 in cultured cardiomyocytes, and tested the hypothesis that mature microRNA-1 can be a substrate for endonuclease activity of proteasome. In our in vitro experiments, we have found that proteasome fraction II is able to degrade both mature and primary but not precursor microRNA-1. However, this in vitro effect was not abolished by chemical inhibitor of proteolytic activities of proteasome. These data let us summarize that proteasome has the complex effect on the level of microRNA-1.

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Acknowledgments

This study was elaborated within the Grant of the European Regional Development Fund—Project FNUSA-ICRC (No. CZ.1.05/1.1.00/02.0123).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Bogomoletz Institute of Physiology, National Academy of Science, Kiev, Ukraine

    Veronika Gurianova, Dmytro Stroy, Victoria Kyrichenko, Alex Moibenko & Victor Dosenko

  2. Department of Cardiovascular Diseases, International Clinical Research Center, St. Anne´s University Hospital, Masaryk University, Pekarska 53, 656 91, Brno, Czech Republic

    Peter Kruzliak

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  1. Veronika Gurianova
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  2. Dmytro Stroy
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  6. Victor Dosenko
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Correspondence to Peter Kruzliak.

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Gurianova, V., Stroy, D., Kruzliak, P. et al. Does proteasome regulate the level of microRNA-1 in cardiomyocytes? Application to anoxia-reoxygenation. Mol Cell Biochem 404, 45–51 (2015). https://doi.org/10.1007/s11010-015-2365-7

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  • DOI: https://doi.org/10.1007/s11010-015-2365-7

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