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CXCL13-CXCR5 axis promotes the growth and invasion of colon cancer cells via PI3K/AKT pathway

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Abstract

CXCL13, an inflammatory factor in the microenvironment, plays a vital role in the progression of inflammatory diseases and tumors. CXCL13 and its receptor CXCR5 have been reported to be associated with poor prognosis of advanced colon cancer. However, the molecular mechanisms of CXCL13-CXCR5 axis in colon cancer remain elusive. The aim of this study was to investigate the role of CXCR5-CXCL13 axis in the growth and invasion of colon cancer cells. Our results showed that CXCL13 promoted the growth, migration, and matrigel invasion of colon cancer cells. Furthermore, CXCL13 increased the expression and secretion of MMP-13, and stimulated the activation of PI3K/AKT pathway. After knockdown of CXCR5 by siRNA, the biological functions of colon cancer cells regulated by CXCL13 were significantly inhibited. In addition, inhibition of PI3K/AKT pathway by specific inhibitor LY294002 suppressed the CXCL13-mediated growth, migration, and invasion of colon cancer cells. Together, our findings suggest that CXCL13-CXCR5 axis promotes the growth, migration, and invasion of colon cancer cells, probably via PI3K/AKT pathway. Thus, CXCL13 may be a useful biomarker for the detection and treatment of colon cancer.

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All authors declare no conflict of interests.

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Authors and Affiliations

  1. Surgiaca Ward 4 (Colorectum & Intestinal Surgery), Shandong Tumor Hospital, Jinan, 250117, Shandong, China

    Zhenyu Zhu & Hongliang Guo

  2. Department of General Surgery, Jinan Military General Hospital, 25 Shifan Road, Jinan, 250031, Shandong, China

    Xukui Zhang & Yinggang Sun

  3. Department of Oncology, Affiliated Hospital of Weifang Medical University, Weifang, 261031, Shandong, China

    Ling Fu

  4. Tianqiao Hospital, Jinan, 250031, Shandong, China

    Ganlin Pan

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  1. Zhenyu Zhu
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  2. Xukui Zhang
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  3. Hongliang Guo
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  4. Ling Fu
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  5. Ganlin Pan
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  6. Yinggang Sun
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Corresponding author

Correspondence to Yinggang Sun.

Additional information

Zhenyu Zhu and Xukui Zhang have contributed equally to this study, and both should be considered as first author.

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Zhu, Z., Zhang, X., Guo, H. et al. CXCL13-CXCR5 axis promotes the growth and invasion of colon cancer cells via PI3K/AKT pathway. Mol Cell Biochem 400, 287–295 (2015). https://doi.org/10.1007/s11010-014-2285-y

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  • DOI: https://doi.org/10.1007/s11010-014-2285-y

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