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Knockdown of ANLN by lentivirus inhibits cell growth and migration in human breast cancer

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Abstract

Anillin (ANLN), an actin-binding protein, is required for cytokinesis. Recently, ANLN has been identified as a biomarker in diverse human cancers; however, the precise role of ANLN in breast cancer remains unclear. In this study, we firstly detected the expression of ANLN in 71 patients with breast cancer by immunohistochemistry, and found ANLN was highly expressed in breast cancer tissues. To evaluate the function of ANLN in breast cancer cells, we employed lentivirus-mediated RNA interference to knock down ANLN expression in two human breast cancer cell lines, MDA-MB-231, and ZR-75-30. Knockdown of ANLN remarkably inhibited the proliferation rate and colony formation ability of both breast cancer cell lines. Moreover, flow cytometry analysis showed that depletion of ANLN in MDA-MB-231 cells blocked the cell cycle progression, with more cells delayed at G2/M phase, due to phosphorylation of Cdc2 and suppression of Cyclin D1. Furthermore, knockdown of ANLN strongly suppressed the migration of breast cancer cells, strengthening the evidence that ANLN could be involved in breast cancer progression. Our results may suggest ANLN as a potential target candidate in breast cancer.

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Acknowledgments

This study was supported by a Grant from the National Natural Science Foundation of China (81372842).

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Authors and Affiliations

  1. Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan, China

    Weibing Zhou, Zhan Wang, Ni Shen, Weiwei Pi & Wuzhong Jiang

  2. Hunan Province Clinic Meditech Research Center for Breast Cancer, Xiangya Hospital, Central South University, Changsha, Hunan, China

    Juan Huang & Yuanping Hu

  3. Center for Molecular Medicine, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008, Hunan, China

    Xiong Li & Lunquan Sun

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  1. Weibing Zhou
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  2. Zhan Wang
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  3. Ni Shen
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  4. Weiwei Pi
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  6. Juan Huang
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  7. Yuanping Hu
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  9. Lunquan Sun
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Correspondence to Lunquan Sun.

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Zhou, W., Wang, Z., Shen, N. et al. Knockdown of ANLN by lentivirus inhibits cell growth and migration in human breast cancer. Mol Cell Biochem 398, 11–19 (2015). https://doi.org/10.1007/s11010-014-2200-6

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  • DOI: https://doi.org/10.1007/s11010-014-2200-6

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