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miR-202 suppresses proliferation and induces apoptosis of osteosarcoma cells by downregulating Gli2

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Abstract

Accumulating data have shown that microRNAs are involved in the pathogenesis of cancer. miR-202 has been confirmed to be downregulated in several types of human cancer. However, the expression and biological role of miR-202 in osteosarcoma (OS) carcinogenesis and progression remain unclear. In this study, we demonstrated that miR-202 expression is significantly decreased in human OS cell lines and specimens. Restoration of miR-202 expression could inhibit OS cell proliferation, induce cell apoptosis, and suppress tumor growth in nude mice models. We subsequently identified the transcription factor Gli2 as a direct target of miR-202. Overexpression of Gli2 blocked the inhibitory function of miR-202. Taken together, our results indicate that miR-202 acts as a novel tumor suppressor to regulate OS cell proliferation and apoptosis through downregulating Gli2 expression.

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Authors and Affiliations

  1. Department of Traumatology, Yantaishan Hospital, Yantai, 264025, Shandong Province, China

    Zhengwen Sun, Huanyu Hong & Haiguang Zhang

  2. Department of Pediatric Orthopedics, Yantaishan Hospital, Yantai, 264025, Shandong Province, China

    Tongqing Zhang

  3. Department of Nursing, Yantaishan Hospital, Yantai, 264025, Shandong Province, China

    Qingxia Liu

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  1. Zhengwen Sun
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  2. Tongqing Zhang
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  3. Huanyu Hong
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  4. Qingxia Liu
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  5. Haiguang Zhang
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Correspondence to Haiguang Zhang.

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Sun, Z., Zhang, T., Hong, H. et al. miR-202 suppresses proliferation and induces apoptosis of osteosarcoma cells by downregulating Gli2. Mol Cell Biochem 397, 277–283 (2014). https://doi.org/10.1007/s11010-014-2195-z

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  • DOI: https://doi.org/10.1007/s11010-014-2195-z

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