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Protective effect of quercetin in ecto-enzymes, cholinesterases, and myeloperoxidase activities in the lymphocytes of rats exposed to cadmium

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Abstract

The ex vivo and in vitro effects of quercetin on NTPDase, adenosine deaminase (ADA), and acetycholinesterase (AChE) activities in lymphocytes, as well as the effects of quercetin on butyrylcholinesterase (BChE) activity in serum and myeloperoxidase (MPO) activity in plasma were determined in rats. For the ex vivo experiment, animals were orally exposed to Cadmium (Cd) for 45 days. Animals were divided into eight groups: saline/ethanol, saline/Querc 5 mg/kg, saline/Querc 25 mg/kg, saline/Querc 50 mg/kg, Cd/ethanol, Cd/Querc 5 mg/kg, Cd/Querc 25 mg/kg, and Cd/Querc 50 mg/kg. The ex vivo data showed an increase in the ATP and ADP hydrolysis and ADA activity in Cd-exposed rats when compared to the control group. The treatment with quercetin 25 and 50 mg/kg prevented this increase in the ATP and ADP hydrolysis, while the treatment with quercetin 5, 25, and 50 mg/kg prevented the increase in the ADA activity. AChE, BChE, and MPO activities ex vivo presented an increase in the Cd-exposed group when compared to the control group, and the treatment with quercetin 5, 25, and 50 mg/kg prevented this increase caused by Cd exposure. The in vitro experiment showed that quercetin 5, 10, 25, or 50 µM decreased the ADA activity proportionally to the increase of the concentrations of quercetin when compared to the control group. Thus, we can suggest that the quercetin is able to modulate NTPDase, ADA, AChE, and MPO activities and contribute to maintain the levels of ATP, adenosine, and acetylcholine normal, respectively, exhibiting potent pro-inflammatory and anti-inflammatory actions.

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Acknowledgments

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Coordenação e Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Federal University of Santa Maria, RS, Brazil, and the FINEP research grant "Rede Instituto Brasileiro de Neurociências (IBNet)”, Instituto Nacional de Ciências Tecnológicas (INCT).

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  1. Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, Santa Maria, RS, 97105-900, Brazil

    Fátima Husein Abdalla, Andréia Machado Cardoso, Jamile Fabbrin Gonçalves, Jucimara Baldissarelli, Caroline Curry Martins, Daniela Zanini, Lizielle Souza de Oliveira, Pauline da Costa, Victor Camera Pimentel, Luciane Belmonte Pereira, Maria Rosa Chitolina Schetinger & Vera Maria Morsch

  2. Instituto Federal de Educação, Ciência e Tecnologia do Triângulo Mineiro, Minas Gerais, CEP, 38600-000, Brazil

    Roberta Schmatz

  3. Departamento de Clínica de Pequenos Animais, Setor de Patologia Clínica Veterinária, Universidade Federal de Santa Maria, Campus Universitário, Camobi, Santa Maria, RS, 97105-900, Brazil

    Cibele Lima Lhamas & Cinthia Melazzo Andrade Mazzanti

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  1. Fátima Husein Abdalla
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Correspondence to Fátima Husein Abdalla or Cinthia Melazzo Andrade Mazzanti.

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Abdalla, F.H., Cardoso, A.M., Schmatz, R. et al. Protective effect of quercetin in ecto-enzymes, cholinesterases, and myeloperoxidase activities in the lymphocytes of rats exposed to cadmium. Mol Cell Biochem 396, 201–211 (2014). https://doi.org/10.1007/s11010-014-2155-7

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