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Lyar, a cell growth-regulating zinc finger protein, was identified to be associated with cytoplasmic ribosomes in male germ and cancer cells

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Abstract

Translational control is a basic mechanism for gene regulation in cells and important for tissue growth and development in mammals. Deregulation of the mechanism thus causes diseases such as cancer. Considering the importance of the ribosome as a factory of polypeptide synthesis, some new factors have been expected to be associated with the ribosome and involved in translational control. Our proteomic survey for these factors identified a zinc finger protein, Lyar, in cytoplasmic ribosomes of the rodent testis. Subcellular fractionation of the testis provided data supporting association of Lyar with ribosomes. Lyar was then suggested to be included in the 60S large subunit, but not in polysomes, by ultracentrifugation of testicular ribosomes. While analysis of tissue distribution of Lyar has indicated its testis-predominant expression, Lyar mRNA was expressed in the cancer cells originated from tissues other than testis, and Lyar promoted proliferation of NIH-3T3 cells. Furthermore, translation was increased by Lyar in vitro, pointing out the first experimental link between this protein and translation. Taken together, Lyar seems to be a new player in translational control and a potential target for cancer therapy.

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Abbreviations

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

MBP:

Maltose-binding protein

MS:

Mass spectrometry

MS/MS:

Tandem mass spectrometry

NCBI:

National center for biotechnology information

PAGE:

Polyacrylamide gel electrophoresis

PCR:

Polymerase chain reaction

PMF:

Peptide mass fingerprint

RT:

Reverse transcription

SDS:

Sodium dodecyl sulfate

TOF:

Time-of-flight

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Acknowledgments

This study was supported in part by a grant-in-aid for Scientific Research (25450514) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8601, Japan

    Kahori Yonezawa, Yoshihiko Sugihara, Kenzi Oshima, Tsukasa Matsuda & Daita Nadano

Authors
  1. Kahori Yonezawa
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  2. Yoshihiko Sugihara
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  3. Kenzi Oshima
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  4. Tsukasa Matsuda
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  5. Daita Nadano
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Corresponding author

Correspondence to Daita Nadano.

Additional information

Kahori Yonezawa, Yoshihiko Sugihara have equally contributed to this work.

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Yonezawa, K., Sugihara, Y., Oshima, K. et al. Lyar, a cell growth-regulating zinc finger protein, was identified to be associated with cytoplasmic ribosomes in male germ and cancer cells. Mol Cell Biochem 395, 221–229 (2014). https://doi.org/10.1007/s11010-014-2128-x

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  • DOI: https://doi.org/10.1007/s11010-014-2128-x

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