Abstract
LPA signaling via LPA receptors [LPA receptor-1 (LPA1)–LPA6] mediates the several cellular responses in cancer cells, including cell motility and invasion. In the present study, to investigate a role of LPA5 in the cell motile and invasive activities of sarcoma cells, LPAR5 knockdown (HOSL5 and HT1080L5) cells were generated from human osteosarcoma HOS and fibrosarcoma HT1080 cells, respectively. In cell motility assays with cell culture inserts, HOSL5 and HT1080L5 cells indicated the high cell motile activities, compared with control cells. The cell invasive activities of HOSL5 and HT1080L5 cells were significantly higher than those of control cells. Moreover, the activities of matrix metalloproteinase (MMP)-2 and MMP-9 were measured by gelatin zymography. MMP-2 was significantly activated in HOSL5 cells, but not MMP-9. The elevated activities of MMP-2 and MMP-9 were found in HT1080L5 cells, in comparison with control cells. These results suggest that LPA signaling via LPA5 negatively regulates the cell motile and invasive activities of human sarcoma cells.
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Abbreviations
- LPA:
-
Lysophosphatidic acid
- LPA5 :
-
LPA receptor-5
- MMP:
-
Matrix metalloproteinase
- RT:
-
Reverse transcription
- PCR:
-
Polymerase chain reaction
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Acknowledgments
This study was supported in part by a Grant-in-Aid (24590493) for Scientific Research (C) from Japan Society for the Promotion of Science, and by a Grant from the Ministry of Health, Labor and Welfare of Japan.
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The authors declare that they have no conflicts of interest.
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Dong, Y., Hirane, M., Araki, M. et al. Lysophosphatidic acid receptor-5 negatively regulates cell motile and invasive activities of human sarcoma cell lines. Mol Cell Biochem 393, 17–22 (2014). https://doi.org/10.1007/s11010-014-2042-2
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DOI: https://doi.org/10.1007/s11010-014-2042-2