Article

Molecular and Cellular Biochemistry

, Volume 344, Issue 1, pp 261-266

MicroRNA-200b regulates cyclin D1 expression and promotes S-phase entry by targeting RND3 in HeLa cells

  • Wei XiaAffiliated withBeijing Institute of Basic Medical Sciences
  • , Jie LiAffiliated withBeijing Institute of Basic Medical Sciences
  • , Liucun ChenAffiliated withBeijing Institute of Basic Medical Sciences
  • , Baochun HuangAffiliated withBeijing Institute of Basic Medical Sciences
  • , Shaohua LiAffiliated withBeijing Institute of Basic Medical Sciences
  • , Guang YangAffiliated withBeijing Institute of Basic Medical Sciences
  • , Hongmei DingAffiliated withBeijing Institute of Basic Medical Sciences
  • , Fang WangAffiliated withBeijing Institute of Basic Medical Sciences
  • , Nongle LiuAffiliated withBeijing Institute of Basic Medical Sciences
    • , Qiang ZhaoAffiliated withBeijing Institute of Basic Medical Sciences
    • , Tao FangAffiliated withBeijing Institute of Basic Medical Sciences
    • , Tao SongAffiliated withChinese PLA General Hospital
    • , Tianyou WangAffiliated withCapital Institute of Pediatrics
    • , Ningsheng ShaoAffiliated withBeijing Institute of Basic Medical Sciences Email author 

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Abstract

MicroRNAs (miRNAs) are endogenous non-coding small RNAs that inhibit gene expression post-transcriptionally. By regulating their target genes, miRNAs play important roles in tumor generation and development. Recently, the mir-200 family was revealed to inhibit the epithelial-mesenchymal transition, which is viewed as an essential step in early tumor metastasis. Here, we used luciferase assays to demonstrate that mir-200b interacts with predicted target sites in the 3′ untranslated region of RND3. In HeLa cells, mir-200b directly reduced the expression of RND3 at the mRNA and protein levels, which thereby promoted expression of the downstream protein cyclin D1 and increased S-phase entry. In conclusion, our study demonstrates a novel role for mir-200b in cell cycle progression and identifies RND3 as a novel mir-200b target.

Keywords

miRNA RND3 Cell cycle CCND1