Molecular and Cellular Biochemistry

, Volume 344, Issue 1, pp 261–266

MicroRNA-200b regulates cyclin D1 expression and promotes S-phase entry by targeting RND3 in HeLa cells

Authors

  • Wei Xia
    • Beijing Institute of Basic Medical Sciences
  • Jie Li
    • Beijing Institute of Basic Medical Sciences
  • Liucun Chen
    • Beijing Institute of Basic Medical Sciences
  • Baochun Huang
    • Beijing Institute of Basic Medical Sciences
  • Shaohua Li
    • Beijing Institute of Basic Medical Sciences
  • Guang Yang
    • Beijing Institute of Basic Medical Sciences
  • Hongmei Ding
    • Beijing Institute of Basic Medical Sciences
  • Fang Wang
    • Beijing Institute of Basic Medical Sciences
  • Nongle Liu
    • Beijing Institute of Basic Medical Sciences
  • Qiang Zhao
    • Beijing Institute of Basic Medical Sciences
  • Tao Fang
    • Beijing Institute of Basic Medical Sciences
  • Tao Song
    • Chinese PLA General Hospital
  • Tianyou Wang
    • Capital Institute of Pediatrics
    • Beijing Institute of Basic Medical Sciences
Article

DOI: 10.1007/s11010-010-0550-2

Cite this article as:
Xia, W., Li, J., Chen, L. et al. Mol Cell Biochem (2010) 344: 261. doi:10.1007/s11010-010-0550-2

Abstract

MicroRNAs (miRNAs) are endogenous non-coding small RNAs that inhibit gene expression post-transcriptionally. By regulating their target genes, miRNAs play important roles in tumor generation and development. Recently, the mir-200 family was revealed to inhibit the epithelial-mesenchymal transition, which is viewed as an essential step in early tumor metastasis. Here, we used luciferase assays to demonstrate that mir-200b interacts with predicted target sites in the 3′ untranslated region of RND3. In HeLa cells, mir-200b directly reduced the expression of RND3 at the mRNA and protein levels, which thereby promoted expression of the downstream protein cyclin D1 and increased S-phase entry. In conclusion, our study demonstrates a novel role for mir-200b in cell cycle progression and identifies RND3 as a novel mir-200b target.

Keywords

miRNA RND3 Cell cycle CCND1

Copyright information

© Springer Science+Business Media, LLC. 2010