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Energetic metabolism during acute stretch-related atrial fibrillation

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Abstract

Background and methods Perturbations in energetic metabolism and impaired atrial contractility may play an important role in the pathogenesis of atrial fibrillation (AF). Besides, atrial stretch is commonly associated with AF. However, the atrial energetics of stretch-related AF are poorly understood. Here, we measured indicators of energy metabolism during acute stretch-related AF. PCr, adenine nucleotides, and derivatives concentrations as well as the activity of the F0F1-ATPase and Na,K-ATPase were obtained after 1 h of stretch and/or AF in isolated rabbit hearts and compared to control hearts without stretch and AF. Results After 1 h of stretch-related AF, the total adenine nucleotides’ pool was significantly lower (42.2 ± 2.6 vs. 63.7 ± 8.3 μmol/g protein in control group, P < 0.05) and the PCr/ATP ratio significantly higher (2.3 ± 0.3 vs. 1.1 ± 0.1 in control group P < 0.05), because of ATP, ADP, and AMP decrease and PCr increase. The sum of high-energy phosphate compounds did not change. There were no significant differences in F0F1-ATPase nor Na,K-ATPase activity between the groups. Conclusions Results show that in this experimental model, acute stretch-related AF induces specific modifications of atrial myocytes energetics that may play a pivotal role in the perpetuation of the arrhythmia.

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Acknowledgments

The authors would like to thank CNRS (UMR 6612) for their support. J. Kalifa was supported by grants ADEREM Marseille, NIH RO1 087055-01, and ACCF/GE Healthcare Career Development Award.

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Authors and Affiliations

  1. Centre de Résonance Magnétique Biologique et Médicale (CRMBM), CNRS UMR n° 6612, Faculté de Médecine de Marseille, Université de la Méditerranée, 27 Bd Jean Moulin, 13385, Marseille Cedex 5, France

    Jérôme Kalifa, Christiane Dalmasso, Patrick Cozzone & Monique Bernard

  2. Département de Cardiologie, CHU, Timone Adultes, 264 Rue Saint Pierre, 13385, Marseille Cedex 05, France

    Jérôme Kalifa, Thierry Chalvidan, Dragos Cozma, Pierre Laurent, Jean-Claude Deharo & Pierre Djiane

  3. Department of Internal Medicine, Center for Arrhythmia Research, University of Michigan, 5025 Venture Drive, Ann Arbor, MI, USA

    Jérôme Kalifa

  4. INSERM U927, Poitiers, France

    Jean-Michel Maixent & David Colin

  5. IUP Génie Physiologique-Informatique, Faculté des Sciences Fondamentales et Appliquées, Université de Poitiers, Bât. Mécanique, 40 Av. du recteur Pineau, 86022, Poitiers Cedex, France

    Jean-Michel Maixent & David Colin

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  1. Jérôme Kalifa
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Correspondence to Monique Bernard.

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Kalifa, J., Maixent, JM., Chalvidan, T. et al. Energetic metabolism during acute stretch-related atrial fibrillation. Mol Cell Biochem 317, 69–75 (2008). https://doi.org/10.1007/s11010-008-9832-3

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