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Attachment of a Frog Skin-Derived Peptide to Functionalized Cerium Oxide Nanoparticles

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Abstract

Peptide Brevinin-2R (B2R) has derived from frog skin secretions and possesses cytotoxic effects on cancer cells. Beside, cerium oxide nanoparticle (CNP) has antioxidant properties and could be used in anticancer studies. The purpose of this study is to investigate antioxidant and cytotoxicity of cerium oxide nanoparticle conjugated with B2R. First, cerium oxide nanoparticles were amine functionalized and peptide attached to it through establishment of peptide bond. Then, (1,1-diphenyl-2-picrylhydrazyl) DPPH and 2, 2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging activities of CNP-B2R were determined. MTT assay were used in order to compare cytotoxicity of CNP- B2R on two different cell lines. HFLF-pI5 cell and A549 cell lines were selected as representative of normal and cancer cells, respectively. Also, the cytotoxic effects of CNP, B2R and CNP-B2R were investigated on A549 cell line. Results of antioxidant evaluations showed that the antioxidant activity of CNP-Peptide increased at higher concentration of CNP-B2R with IC50 of 0.2 and 0.54 (mg/mL) for DPPH and ABTS assays, respectively. The results of cytotoxicity effects showed that CNP-B2R was more potent in killing tumor cell line in comparison with normal cell line. Cytotoxicity of CNP, B2R and CNP-B2R demonstrated that CNP-B2R and B2R had the lower cell viability effects compared to CNP. Our findings showed cytotoxicity of CNP-B2R against cancer cell lines in comparison with normal cells indicating the potential anticancer properties of CNP-B2R.

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Acknowledgments

This work was supported by Mashhad Branch, Islamic Azad University, Mashhad, Iran, (Grant No. 92428.304.73). The authors are also grateful to Ferdowsi University of Mashhad, Mashhad, Iran, and Mashhad University of medical Sciences, Mashhad, Iran.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Mashhad Branch, Islamic Azad University, Mashhad, Iran

    Masoud Homayouni-Tabrizi

  2. Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

    Ahmad Asoodeh

  3. Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Mashreghi

  4. Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

    Mahere Rezazade Bazaz

  5. Targeted Drug Delivery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Reza Kazemi Oskuee

  6. Nuclear Medicine Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Majid Darroudi

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  1. Masoud Homayouni-Tabrizi
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  2. Ahmad Asoodeh
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  3. Mohammad Mashreghi
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  4. Mahere Rezazade Bazaz
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  5. Reza Kazemi Oskuee
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  6. Majid Darroudi
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Correspondence to Masoud Homayouni-Tabrizi.

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Masoud Homayouni-Tabrizi, Ahmad Asoodeh, Mohammad Mashreghi, Mahere Rezazade Bazaz, Reza Kazemi Oskuee, and Majid Darroudi declare that they have no conflict of interest.

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This article does not contain any studies with human or animal subjects performed by any of the authors.

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Homayouni-Tabrizi, M., Asoodeh, A., Mashreghi, M. et al. Attachment of a Frog Skin-Derived Peptide to Functionalized Cerium Oxide Nanoparticles. Int J Pept Res Ther 22, 505–510 (2016). https://doi.org/10.1007/s10989-016-9531-y

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  • DOI: https://doi.org/10.1007/s10989-016-9531-y

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