Abstract
Insulin-like growth factor II (IGF-II) is a protein with high structural and sequence similarity to insulin. Unlike insulin, it binds both the type 1 IGF receptor and the exon 11- isoform of the insulin receptor with high affinity. The overexpression and up regulation of IGF-II has been associated with the progression of various forms of cancer. The exact binding mechanism of IGF-II to its high affinity receptors is still not completely understood. Herein we describe the successful synthesis of a novel fluorescent IGF-II protein (F19Cou IGF-II), where residue 19 (phenylalanine) has been replaced by a fluorescent chromophore (coumaryl glycine). This novel coumaryl IGF-II analogue will be a useful tool for analysing the receptor interaction mechanisms in future studies.
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Acknowledgments
The authors would like to acknowledge Clair Alvino for helpful discussions and assistance with competitive binding assays and the Australian Research Council (ARC) for funding.
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Cottam, J.M., Scanlon, D.B., Karas, J.A. et al. Chemical Synthesis of a Fluorescent IGF-II Analogue. Int J Pept Res Ther 19, 61–69 (2013). https://doi.org/10.1007/s10989-012-9339-3
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DOI: https://doi.org/10.1007/s10989-012-9339-3