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Inhibiting Effects of a Cyclic Peptide CNGRC on Proliferation and Migration of Tumor Cells In Vitro

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Abstract

The cyclic peptide Cys-Asn-Gly-Arg-Cys (CNGRC) has previously been demonstrated as a tumor vasculature-homing peptide, which can specifically bind to CD13/aminopeptidase N in vivo. However, the effect of the peptide (CNGRC) binding to tumor cells in vitro has not yet been reported. In this study, CNGRC and an irrelevant linear control peptide (SVSVG) were employed to investigate the specific binding properties and other cellular influences in vitro. Immunofluorescence revealed that the peptide CNGRC demonstrated high specificities to the cells MDA-MB-435S, A549, MDA-MB-231, SK-OV-3 and EA.hy926, respectively. The cell viability assay indicated that CNGRC inhibited the proliferation of tumor cells at 24, 48 and 72 h. Furthermore, the peptide efficiently inhibited the migration of tumor cells, but promoted the migration of the human umbilical vein cell line. These results demonstrate that the synthetic peptide CNGRC can bind to the tumor cells without aminopeptidase N (CD13) expressed on the membranes. Therefore, it is supposed that the mechanism of the peptide binding to tumor cells in vitro may be different from that in vivo.

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Abbreviations

CNGRC:

Cys-Asn-Gly-Arg-Cys

CD13:

Aminopeptidase N

FITC:

Fluorescein isothiocyanate

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

ECM:

Extracellular matrix

isoD:

Isoaspartate

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Acknowledgments

This work has been supported by the National Natural Science Foundation of China (project Nos. 60871062 and 50873066). The supports of Sichuan Province through a Science Fund for Distinguished Young Scholars of Sichuan Province (08ZQ026-007) and Key Technologies Research and Development Program of Sichuan Province (2010SZ0088, 2008SZ0021 and 2006Z08-001-1) are also acknowledged with gratitude.

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Authors and Affiliations

  1. College of Materials Science and Engineering, Sichuan University, No. 24, South 1st Section, 1st Ring Road, Chengdu, 610065, Sichuan, People’s Republic of China

    Yan Wei, Guangfu Yin, Hao Yin, Chuying Ma, Zhongbing Huang, Xiaoming Liao, Yadong Yao & Xianchun Chen

  2. Chien-shiung institute of technology, 215411, Taicang, Jiangsu, People’s Republic of China

    Danhong Yan

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  1. Yan Wei
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  2. Guangfu Yin
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  3. Hao Yin
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  4. Danhong Yan
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  5. Chuying Ma
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  7. Xiaoming Liao
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  9. Xianchun Chen
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Correspondence to Guangfu Yin.

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Wei, Y., Yin, G., Yin, H. et al. Inhibiting Effects of a Cyclic Peptide CNGRC on Proliferation and Migration of Tumor Cells In Vitro. Int J Pept Res Ther 19, 163–173 (2013). https://doi.org/10.1007/s10989-012-9327-7

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  • DOI: https://doi.org/10.1007/s10989-012-9327-7

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