International Journal of Peptide Research and Therapeutics

, Volume 18, Issue 4, pp 353–360

Structure and ACE-Inhibitory Activity of Peptides Derived from Hen Egg White Lysozyme

  • Mina Memarpoor-Yazdi
  • Ahmad Asoodeh
  • JamshidKhan Chamani
Article

DOI: 10.1007/s10989-012-9311-2

Cite this article as:
Memarpoor-Yazdi, M., Asoodeh, A. & Chamani, J. Int J Pept Res Ther (2012) 18: 353. doi:10.1007/s10989-012-9311-2

Abstract

Angiotensin I-converting enzyme plays an important role in hypertension and therefore its inhibition is considered to be a useful procedure in the prevention of hypertension. Two novel ACE inhibitory peptides were purified and identified from the papain-trypsin hydrolysate of hen egg white lysozyme using reverse phase-high performance liquid chromatography. The sequences of identified peptides were NTDGSTDYGILQINSR (MW: 1,753.98 ± 0.5 Da) and VFGR (MW: 459.26 ± 0.5 Da), which were named F2 and F9 peptide, respectively. Analyses of the far-UV CD spectra of ACE in the absence and presence of the F2 peptide revealed ACE secondary structural changes. In the presence of the F2 peptide, a loss of helical content of ACE was observed, which can lead to decrease of the enzymatic activity. Lineweaver–Burk plots show that the identified peptides both act as non-competitive ACE inhibitors. These findings would be helpful on the understanding of interaction between ACE and its inhibitory peptides.

Keywords

RP-HPLCACE inhibitory activityInhibition patternCircular dichroism spectroscopy

Abbreviations

ACE

Angiotensin I-converting enzyme

FAPGG

N-[3-(2-furylacryloyl-Phe-Gly–Gly)]

RP-HPLC

Reverse phase high-performance liquid chromatography

HEWL

Hen egg white lysozyme

CD

Circular dichroism

MALDI-TOF

Matrix-assisted laser desorption/ionization time of flight

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Mina Memarpoor-Yazdi
    • 1
  • Ahmad Asoodeh
    • 2
    • 3
  • JamshidKhan Chamani
    • 1
  1. 1.Department of Biology, Faculty of Sciences, Mashhad BranchIslamic Azad UniversityMashhadIran
  2. 2.Department of Chemistry, Faculty of SciencesFerdowsi University of MashhadMashhadIran
  3. 3.Cellular and Molecular Research Group, Institute of BiotechnologyFerdowsi University of MashhadMashhadIran