Abstract
Gastric cancer is the second cause of the common cancer deaths worldwide. Basic fibroblast growth factor (bFGF) was up-regulated in patients with gastric cancer and was regarded as a potential therapeutic target. We have previously obtained a high-affinity bFGF-binding peptide (named P7) from the phage display random heptapeptide library. In this study, we first proved that P7 peptides inhibited proliferation of gastric cancer cell lines including SGC-7901, AGS, and BGC-823 cells stimulated with bFGF. Further investigations with SGC-7901 cells revealed that the mechanisms of P7 peptides inhibiting bFGF-stimulated cell proliferation in vitro involved cell cycle arrest at the G0/G1 phase, blockade of the activation of Erk and P38 cascades, and suppression of bFGF internalization. The results suggested that the bFGF-binding peptide may have therapeutic potential in gastric cancer therapy.
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Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (30973671, 81071800), the Natural Science Foundation of Zhejiang Province of China (Y2090292), the Natural Science Foundation of Guangdong Province of China (9151064001000031), the Science and Technology Planning Project of Wenzhou (Y20090244), the Fundamental Research Funds for the Central Universities (X. P. Wu), Guangdong Provincial “Thousand-Hundred-Ten Talent Project” (X. P. Wu), and Guangdong Provincial Key Discipline in Biochemistry and Molecular biology.
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S. Gao and Y. Yu contributed equally to this work.
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Gao, S., Yu, Y., Lai, X. et al. Anti-Proliferation Effect of a Short Peptide on bFGF-Induced Human Gastric Cancer Cells. Int J Pept Res Ther 18, 299–304 (2012). https://doi.org/10.1007/s10989-012-9301-4
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DOI: https://doi.org/10.1007/s10989-012-9301-4