Abstract
In an effort to probe the reaction of antibiotic hydrolysis catalyzed by B3 metallo-β-lactamase (MβL), the thermodynamic parameters of penicillin G hydrolysis catalyzed by MβL L1 from Stenotrophomonas maltophilia were determined by microcalorimetric method. The values of activation free energy ΔG θ≠ are 88.26, 89.44, 90.49, and 91.57 kJ mol−1 at 293.15, 298.15, 303.15, and 308.15 K, respectively, activation enthalpy ΔH θ≠ is 24.02 kJ mol−1, activation entropy ΔS θ≠ is −219.2511 J mol−1 K−1, apparent activation energy E is 26.5183 kJ mol−1, and the reaction order is 1.0. The thermodynamic parameters reveal that the penicillin G hydrolysis catalyzed by MβL L1 is an exothermic and spontaneous reaction.
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Acknowledgements
This work was supported by grants (to K. W. Y) from National Natural Science Fund of China (20972127), Doctoral Fund of China (200806970005), Natural Science Fund of Shaanxi Province (2009JM2002) and Key Fund for International Cooperation of Shaanxi Province (2010KW-16).
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Gao, HZ., Yang, Q., Yan, XY. et al. Exploring antibiotic resistant mechanism by microcalorimetry. J Therm Anal Calorim 107, 321–324 (2012). https://doi.org/10.1007/s10973-011-1362-7
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DOI: https://doi.org/10.1007/s10973-011-1362-7