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Synthesis and characterization of immobilized white-rot fungus Trametes versicolor in sol–gel ceramics

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Abstract

Trametes versicolor (TV) cells were embedded in ceramic matrices (TV Biocers) through sol–gel method using tetraethyl orthosilicate as a silica precursor. The characterization of the free silica and TV Biocers was carried out by using scanning electron microscope, transmission electron microscope, Fourier transform infrared spectrophotometer, nitrogen adsorption–desorption measurement and catalytic activity assay. The performance of the TV Biocers as biocatalysts was evaluated using methylene blue (MB) and malachite green (MG) dyes as model emerging organic micropollutants. It was observed that the dye removal performance η (mmol g−1) of the TV Biocers for MB and MG, respectively, was 7.400 and 5.569 mmol g−1 which was 18 and 128 % higher than the TV Biocers calculated values obtained from the free silica and free TV cells. These results demonstrated that the TV Biocers can offer better dye removal performance through a combination of adsorption and degradation processes. This is the first reported study on the immobilization of the TV cells in silica matrices, and further study is underway to improve their properties toward its applications for removal of emerging organic micropollutants.

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References

  1. Furusaki S (1988) Engineering aspects of immobilized biocatalysts. Jpn J Chem Eng 21:219–230

    Article  Google Scholar 

  2. Caniato R, Piovan A, Filippini R, Innocenti G, Cappelletti EM (1995) Immobilization of plant cells in hybrid sol–gel materials. J Sol-Gel Sci Technol 7:87–97

    Google Scholar 

  3. Pope EJA, Broun K, Peterson CM (1997) Bioartificial organ 1: silica gel encapsulated pancreatic islets for the treatment of diabetes mellitus. J Sol-Gel Sci Technol 8:635–639

    Google Scholar 

  4. Karout A, Buisson P, Perrard A, Pierre AC (2005) Shaping and mechanical reinforcement of silica aerogel biocatalysts with ceramic fiber felts. J Sol-Gel Sci Technol 36:163–171

    Article  Google Scholar 

  5. Kudanga T, Nyanhongo GS, Guebitz GM, Burton S (2011) Potential applications of laccase-mediated coupling and grafting reaction: a review. Enzyme Microb Technol 48:195–208

    Article  Google Scholar 

  6. Couto SR, Herrera JLT (2006) Industrial and biotechnological applications of laccases: a review. Biotechnol Adv 24:500–513

    Article  Google Scholar 

  7. Türker M, Kennedy JF (1992) Immobilized whole cell systems: engineering considerations. Biopapers J 12:9–28

    Google Scholar 

  8. Davis SA, Burkett SL, Mendelson NH, Mann S (1997) Bacterial templating of ordered macrostructures in silica and silica-surfactant mesophases. Nature 385:420–423

    Article  Google Scholar 

  9. Marco-Urrea E, Pérez-Trujilli M, Vicent T, Caminal G (2009) Ability of white-rot fungi to remove selected pharmaceutical and identification of degradation products of ibuprofen by Trametes versicolor. Chemosphere 74:765–772

    Article  Google Scholar 

  10. Baldrian P (2006) Fungal laccases—occurrence and properties. FEMS Microbiol Rev 30:215–242

    Article  Google Scholar 

  11. Mohidem NA, Mat HB (2009) The catalytic activity of laccase immobilized in sol–gel silica. J Appl Sci 9:3141–3145

    Article  Google Scholar 

  12. Segura F, Durany G, Lozano R, Huguet V (2008) Detoxification pretreatment of black liquor derived from non-wood feedstock with white-rot fungi. Environ Technol 14:681–687

    Article  Google Scholar 

  13. Majeau JA, Brar SK, Tyagi RD (2010) Laccase for removal of recalcitrant and emerging pollutants. Bioresour Technol 101:2331–2350

    Article  Google Scholar 

  14. Mohidem NA, Mat HB (2011) Catalytic activity and stability of laccase entrapped in sol–gel silica with additives. J Sol-Gel Sci Technol 114:472–477

    Google Scholar 

  15. Minussi RC, Pastore GM, Durán N (2002) Potential application of laccase in the food industry. Trends Food Sci Technol 13:205–216

    Article  Google Scholar 

  16. Brijwani K, Rigdon A, Vadlani PV (2010) Fungal laccases: production, function and applications in food processing. Enzyme Res. doi:10.4061/2010/149748

    Google Scholar 

  17. Fernández-Fernández M, Sanromán MÁ, Moldes D (2013) Recent developments and applications of immobilized laccase. Biotechnol Adv 31:1808–1825

    Article  Google Scholar 

  18. Meunier CF, Dandoy P, Su BL (2010) Encapsulation of cells within silica matrices: towards a new advance in the conception of living hybrid materials. J Colloid Interface Sci 342:211–224

    Article  Google Scholar 

  19. Soltmann U, Böttcher H (2008) Utilization of sol–gel ceramics for the immobilization of living microorganism. J Sol-Gel Sci Technol 48:66–72

    Article  Google Scholar 

  20. Viswanath B, Subhosh Chandra M, Pallave H, Rajasekhar Reddy B (2008) Screening and assessment of laccase producing fungi isolated from different environmental samples. Afr J Biotechnol 7:1129–1133

    Google Scholar 

  21. Mansoori GA (2010) Synthesis of nanoparticles by fungi. US Patent 12, 511, 800. Chicago, IL: US

  22. Ding WK, Shah NP (2009) Effect of homogenization techniques on reducing the size of microcapsules and the survival of probiotic bacteria therein. J Food Sci. doi:10.1111/j.17503841.2009.01195.x

    Google Scholar 

  23. Burden D (2012) Guide to the disruption of biological samples. Random Prim 12:1–25

    Google Scholar 

  24. Ho CW, Tan WS, Yap WB, Ling TC, Tey BT (2008) Comparative evaluation of different cell disruption methods for the release of recombinant hepatitis B core antigen from Escherichia coli. Biotechnol Bioprocess Eng 13:577–583

    Article  Google Scholar 

  25. Webb PA, Orr C (1997) Analytical methods in fine particle technology. Micromeritics Instrument Corp., USA

    Google Scholar 

  26. Gao S, Wang Y, Diao X, Luo G, Dai Y (2010) Effect of pore diameter and cross-linking method on the immobilization efficiency of Candida rugosa lipase in SBA-15. Bioresour Technol 101:3830–3837

    Article  Google Scholar 

  27. Pérez-Quintanilla D, Hierro ID, Fajardo M, Sierra I (2006) 2-Mercaptothiazoline modified mesoporous silica for mercury removal from aqueous media. J Hazard Mater 134:245–246

    Article  Google Scholar 

  28. Vera-Avila LE, Morales-Zamudio E, Garcia-Camacho MK (2004) Activity and reusability of sol–gel encapsulated a-amylase and catalase performance in flow-through systems. J Sol-Gel Sci Technol 30:197–204

    Article  Google Scholar 

  29. Dickson DJ, Ely RL (2011) Evaluation of encapsulation stress and the effect of additives on viability and photosynthetic activity of Synechocystis sp. PCC 6803 encapsulated in silica gel. Appl Microbiol Biotechnol 91:1633–1646

    Article  Google Scholar 

  30. Ohkuma M, Maeda Y, Johjima T, Kudo T (2001) Lignin degradation and roles of white rot fungi: study on an efficient symbiotic system in fungus-growing termites and its application to bioremediation. RIKEN Rev Foc Econ Sci Res 42:39–42

    Google Scholar 

  31. Siripong P, Oraphin B, Sanro T, Duanporn P (2009) Screening of fungi from natural sources in Thailand for degradation of polychlorinated hydrocarbons. Am Eur J Agric Environ Sci 5:466–472

    Google Scholar 

  32. Ogugbue CJ, Sawidis T (2011) Bioremediation and detoxification of synthetic wastewater containing triarylmethane dyes by Aromoas hydrophila isolated from industrial effluents. Biotechnol Res Int. doi:10.4061/2011/967925

    Google Scholar 

  33. Rajaguru P, Kalaiselvi K, Palanivel M, Subburam V (2000) Biodegradation of azo dyes in a sequential anaerobic-aerobic system. Appl Microbiol Biotechnol 54:268–273

    Article  Google Scholar 

  34. Bao-e W, Yong-you H (2006) Comparison of four supports for adsorption of reactive dyes by immobilized Aspergillus fumigatus beads. J Environ Sci 19:451–457

    Google Scholar 

  35. Mohidem NA, Mat HB (2012) The catalytic activity enhancement and biodegradation potential of free laccase and novel sol-gel laccase in non-conventional solvents. Bioresour Technol 114:472–477

    Article  Google Scholar 

  36. Mansor AF, Mohidem NA, Wan Mohd Zawawi WNI, Othman NS, Endud S, Mat HB (2015) Preparation and characterization of in situ entrapment of laccase in silica microparticles via an ambient drying procedure. J Sol Gel Sci Technol. doi:10.1007/s1097101537037

    Google Scholar 

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Acknowledgments

The financial supports from the Ministry of Higher Education (MOHE), Malaysia, for the MyBrain15 scholarships and the Fundamental Research Grant Scheme (FRGS Grant No. 4F218) are gratefully acknowledged.

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Authors and Affiliations

  1. Advanced Materials and Process Engineering Laboratory, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, UTM, 81310, Skudai, Johor, Malaysia

    Wan Nurul Izyani Wan Mohd Zawawi, Azmi Fadziyana Mansor, Nurul Sakinah Othman, Nur Atikah Mohidem & Hanapi Mat

  2. Novel Materials Research Group, Frontier Materials Research Alliance, Universiti Teknologi Malaysia, UTM, 81310, Skudai, Johor, Malaysia

    Nik Ahmad Nizam Nik Malek & Hanapi Mat

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  1. Wan Nurul Izyani Wan Mohd Zawawi
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  2. Azmi Fadziyana Mansor
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  3. Nurul Sakinah Othman
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  4. Nur Atikah Mohidem
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  5. Nik Ahmad Nizam Nik Malek
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  6. Hanapi Mat
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Correspondence to Hanapi Mat.

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The authors of this manuscript declare no relationships with any companies, whose products or services may be related to the subject matter of the article. This research does not involve human and/or animal participants, and thus, informed consent is negligible.

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Wan Mohd Zawawi, W.N.I., Mansor, A.F., Othman, N.S. et al. Synthesis and characterization of immobilized white-rot fungus Trametes versicolor in sol–gel ceramics. J Sol-Gel Sci Technol 77, 28–38 (2016). https://doi.org/10.1007/s10971-015-3824-z

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  • DOI: https://doi.org/10.1007/s10971-015-3824-z

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