Short Communication

Journal of Structural and Functional Genomics

, Volume 13, Issue 1, pp 15-26

Crystal structures of putative phosphoglycerate kinases from B. anthracis and C. jejuni

  • Heping ZhengAffiliated withDepartment of Molecular Physiology and Biological Physics, University of VirginiaCenter for Structural Genomics of Infectious Diseases (CSGID)
  • , Ekaterina V. FilippovaAffiliated withDepartment of Molecular Pharmacology and Biological Chemistry, Northwestern University Feinberg School of MedicineCenter for Structural Genomics of Infectious Diseases (CSGID)
  • , Karolina L. TkaczukAffiliated withDepartment of Molecular Physiology and Biological Physics, University of VirginiaCenter for Structural Genomics of Infectious Diseases (CSGID)
  • , Piotr DworzynskiAffiliated withDepartment of Molecular Physiology and Biological Physics, University of VirginiaCenter for Structural Genomics of Infectious Diseases (CSGID)
  • , Maksymilian ChruszczAffiliated withDepartment of Molecular Physiology and Biological Physics, University of VirginiaCenter for Structural Genomics of Infectious Diseases (CSGID)
  • , Przemyslaw J. PorebskiAffiliated withDepartment of Molecular Physiology and Biological Physics, University of VirginiaCenter for Structural Genomics of Infectious Diseases (CSGID)
  • , Zdzislaw WawrzakAffiliated withCenter for Structural Genomics of Infectious Diseases (CSGID)Northwestern University, Synchrotron Research Center
  • , Olena OnopriyenkoAffiliated withBanting and Best Department of Medical Research, University of TorontoCenter for Structural Genomics of Infectious Diseases (CSGID)
  • , Marina KudritskaAffiliated withBanting and Best Department of Medical Research, University of TorontoCenter for Structural Genomics of Infectious Diseases (CSGID)
    • , Sarah GrimshawAffiliated withJ. Craig Venter InstituteCenter for Structural Genomics of Infectious Diseases (CSGID)
    • , Alexei SavchenkoAffiliated withBanting and Best Department of Medical Research, University of TorontoCenter for Structural Genomics of Infectious Diseases (CSGID)
    • , Wayne F. AndersonAffiliated withDepartment of Molecular Pharmacology and Biological Chemistry, Northwestern University Feinberg School of MedicineCenter for Structural Genomics of Infectious Diseases (CSGID)
    • , Wladek MinorAffiliated withDepartment of Molecular Physiology and Biological Physics, University of VirginiaCenter for Structural Genomics of Infectious Diseases (CSGID) Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Phosphoglycerate kinase (PGK) is indispensable during glycolysis for anaerobic glucose degradation and energy generation. Here we present comprehensive structure analysis of two putative PGKs from Bacillus anthracis str. Sterne and Campylobacter jejuni in the context of their structural homologs. They are the first PGKs from pathogenic bacteria reported in the Protein Data Bank. The crystal structure of PGK from Bacillus anthracis str. Sterne (BaPGK) has been determined at 1.68 Å while the structure of PGK from Campylobacter jejuni (CjPGK) has been determined at 2.14 Å resolution. The proteins’ monomers are composed of two domains, each containing a Rossmann fold, hinged together by a helix which can be used to adjust the relative position between two domains. It is also shown that apo-forms of both BaPGK and CjPGK adopt open conformations as compared to the substrate and ATP bound forms of PGK from other species.

Keywords

Carbohydrate degradation Glycolysis PGK Phosphoglycerate kinase Pathogenic organism Anthrax Gastroenteritis Guillain–Barré syndrome Rossmann fold Bacillus anthracis Campylobacter jejuni