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Biodistribution of ultra small superparamagnetic iron oxide nanoparticles in BALB mice

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Abstract

Recently ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles (NPs) have been widely used for medical applications. One of their important applications is using these particles as MRI contrast agent. While various research works have been done about MRI application of USPIOs, there is limited research about their uptakes in various organs. The aim of this study was to evaluate the biodistribution of dextran coated iron oxide NPs labelled with 99mTc in various organs via intravenous injection in Balb/c mice. The magnetite NPs were dispersed in phosphate buffered saline and SnCl2 which was used as a reduction reagent. Subsequently, the radioisotope 99mTc was mixed directly into the reaction solution. The labeling efficiency of USPIOs labeled with 99mTc, was above 99 %. Sixty mice were sacrificed at 12 different time points (From 1 min to 48 h post injections; five mice at each time). The percentage of injected dose per gram of each organ was measured by direct counting for 19 harvested organs of the mice. The biodistribution of 99mTc-USPIO in Balb/c mice showed dramatic uptake in reticuloendothelial system. Accordingly, about 75 percent of injected dose was found in spleen and liver at 15 min post injection. More than 24 % of the NPs remain in liver after 48 h post-injection and their clearance is so fast in other organs. The results suggest that USPIOs as characterized in our study can be potentially used as contrast agent in MR Imaging, distributing reticuloendothelial system specially spleen and liver.

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Authors and Affiliations

  1. Department of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran

    Saeed Shanehsazzadeh & Mohammad Ali Oghabian

  2. Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran

    Saeed Shanehsazzadeh & Mohammad Ali Oghabian

  3. Nuclear Science Faculty, Nuclear Science & Technology Research Institute, Tehran, Iran

    Fariba Johari Daha

  4. Department of Medicinal Chemistry, Faculty of Pharmacy and Drug Design and Development Research Center, Tehran University of Medical Science, Tehran, Iran

    Massoud Amanlou

  5. Experimental Radiation Oncology, Cancer Pathology & Cell Biology Laboratory, Ingham Institute of Applied Medical Research, Discipline of Pathology, School of Medicine, University of Western Sydney, NSW, Australia

    Barry J. Allen

Authors
  1. Saeed Shanehsazzadeh
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  2. Mohammad Ali Oghabian
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  4. Massoud Amanlou
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Correspondence to Mohammad Ali Oghabian.

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Shanehsazzadeh, S., Oghabian, M.A., Daha, F.J. et al. Biodistribution of ultra small superparamagnetic iron oxide nanoparticles in BALB mice. J Radioanal Nucl Chem 295, 1517–1523 (2013). https://doi.org/10.1007/s10967-012-2173-4

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  • DOI: https://doi.org/10.1007/s10967-012-2173-4

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