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Bi-layered porous constructs of PCL-coated 45S5 bioactive glass and electrospun collagen-PCL fibers

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Abstract

A simple yet promising approach to construct bi-layered scaffolds using bioactive ceramics and biodegradable polymers is presented. This method involves two versatile fabrication techniques used in the field of TE: foam replication process and electrospinning. By the foam replication method, three-dimensional 45S5 bioactive glass (BG)-based scaffolds with high porosity, in the range of 95.8 ± 0.9 %, were produced. To improve the mechanical properties of the BG scaffolds, dip-coating using polycaprolactone (PCL) was performed, which led to a significant increase in the compressive strength of the scaffolds. In order to develop a bi-layered construct, bead-less submicrometric fibers of collagen-PCL were electrospun over the PCL-coated BG scaffolds. Surface morphology, surface properties and mechanical strength of the bi-layered construct were evaluated using scanning electron microscopy analysis, contact angle measurements and compressive strength testing, respectively. In vitro degradation of the collagen-PCL fibers in phosphate buffered saline and in vitro bioactivity of the bi-layered constructs in simulated body fluid were investigated. Formation of hydroxyapatite on the PCL-coated BG and along the morphology of the collagen-PCL fibers was ascertained using different characterization techniques. The bi-layered construct is intended for interface tissue engineering applications where the PCL-coated BG scaffold, which is highly bioactive, can serve as a support for the bone side and the composite collagen-PCL submicrometric fibers are intended for the cartilage side.

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Acknowledgments

The authors would like to acknowledge the European Commission funding under the 7th Framework Programme (Marie Curie Initial Training Networks; Grant Number: 289958, Bioceramics for bone repair). The authors thank Dr. Patcharakamon Nooeaid, Ms Yaping Ding, Mr Wei Li and Dr Menti Goudouri (Institute of Biomaterials, University of Erlangen-Nuremberg) for experimental support.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058, Erlangen, Germany

    Preethi Balasubramanian & Aldo R. Boccaccini

  2. Department of Materials Science and Engineering, Institute of Polymer Materials, University of Erlangen-Nuremberg, Martenstr. 7, 91058, Erlangen, Germany

    Judith A. Roether & Dirk W. Schubert

  3. Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, University Hospital Erlangen, University of Erlangen-Nuremberg, Erlangen, Germany

    Justus P. Beier

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  1. Preethi Balasubramanian
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  2. Judith A. Roether
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  5. Aldo R. Boccaccini
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Correspondence to Aldo R. Boccaccini.

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Balasubramanian, P., Roether, J.A., Schubert, D.W. et al. Bi-layered porous constructs of PCL-coated 45S5 bioactive glass and electrospun collagen-PCL fibers. J Porous Mater 22, 1215–1226 (2015). https://doi.org/10.1007/s10934-015-9998-5

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  • DOI: https://doi.org/10.1007/s10934-015-9998-5

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