The Protein Journal

, Volume 28, Issue 3, pp 117–123

Interaction of Curcumin and Diacetylcurcumin with the Lipocalin Member β-Lactoglobulin

Authors

  • Fakhrossadat Mohammadi
    • Laboratory of Biophysical Chemistry, Department of ChemistryUniversity of Isfahan
    • Laboratory of Biophysical Chemistry, Department of ChemistryUniversity of Isfahan
  • Adeleh Divsalar
    • Institute of Biochemistry and BiophysicsUniversity of Tehran
  • Khosro Mohammadi
    • Department of Chemistry, College of SciencesPersian Gulf University
  • Ali Akbar Saboury
    • Institute of Biochemistry and BiophysicsUniversity of Tehran
Article

DOI: 10.1007/s10930-009-9171-6

Cite this article as:
Mohammadi, F., Bordbar, A., Divsalar, A. et al. Protein J (2009) 28: 117. doi:10.1007/s10930-009-9171-6

Abstract

The binding of curcumin (CUR) and diacetylcurcumin (DAC) to bovine beta-lactoglobulin (BLG) genetic variant B was investigated by fluorescence and circular dichroism techniques. The binding parameters including number of substantive binding sites and the binding constants have been evaluated by fluorescence quenching method. The distance (r) between donor (BLG) and acceptor (CUR and DAC) was obtained according to the Förster’s theory of non-radiative energy transfer. The far-UV circular dichroism spectra were used to investigate the possible changes in the secondary structure of BLG in the presence of CUR and DAC and showed that these two ligands change the α-helix and random coil contents of this protein to some extent. The visible circular dichroism spectra indicated that the optical activity during the ligand binding was observed due to the induced-protein chirality. All of the achieved results suggested the important role of the phenolic OH group of CUR in the binding process resulted in more affinity of CUR than DAC for binding to BLG.

Keywords

Curcumin Diacetylcurcumin Bovine β-lactoglobulin Fluorescence Circular dichroism

Abbreviations

CUR

Curcumin

DAC

Diacetylcurcumin

BLG

Beta-lactoglobulin

CD

Circular dichroism

Asp

Aspartic acid

Ala

Alanine

Gly

Glycine

Val

Valine

HSA

Human serum albumin

BSA

Bovine serum albumin

Copyright information

© Springer Science+Business Media, LLC 2009