Abstract
Cassava starch waste hydrolysates (CSWHs) with different degrees of polymerisation, i.e., CSWHs-1, CSWHs-2 and CSWHs-3, were prepared through the hydrolysis of cassava starch waste with thermostable a-amylase from Thermococcus sp. HJ21. The prepared CSWHs were then used as a carbon source for curdlan production with Alcaligenes faecalis ATCC 31749. The amount of curdlan produced and the glucosyltransferase activity during curdlan synthesis increased more obviously when CSWHs-2 was used as the carbon source than when glucose was used. Using both carbon sources, the maximum curdlan production was observed at day 5, and the maximum glucosyltransferase activity was observed at day 4. Glucosyltransferase activity decreased thereafter, and biomass continued to increase until the end of the experiment (day 6). Results indicated that the enhanced curdlan production with CSWHs as the carbon source was highly correlated with glucosyltransferase activity.
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Acknowledgements
This work was financially supported by the Public Science and Technology Research Fund Projects of Ocean (201205020-8), the National Natural Science Foundation of China (31271929, 31471719), the National Key Technologies R and D Programme of China (2012BAC07B03), the Priority Academic Programme Development of Jiangsu Higher Education Institutions and Open subject from Jiangsu Marine Resources Development Research Institute (JSIMR201512).
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Wu, S., Lu, M., Fang, Y. et al. Production of Curdlan Grown on Cassava Starch Waste Hydrolysates. J Polym Environ 26, 33–38 (2018). https://doi.org/10.1007/s10924-016-0912-2
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DOI: https://doi.org/10.1007/s10924-016-0912-2