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Sulfated Polysaccharides from Tunisian Fish Skins: Antioxidant, DNA Damage Protective Effect and Antihypertensive Activities

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Abstract

Sulfated polysaccharides were isolated from two Tunisian fish skins grey triggerfish (Balistes capriscus) (GTSP) and smooth hound (Mustelus mustelus) (SHSP). Their chemical and physical characteristics were investigated. The Analysis of surface morphology by scanning electron microscopy of both sulfated polysaccharides displayed the same shape with netted structure. The antioxidant activities of GTSP and SHSP were evaluated using various in vitro antioxidant assays: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity, reducing power, β-carotene bleaching inhibition assay (IC50 GTSP = 0.5 mg mL−1 and IC50 SHSP = 0.6 mg mL−1) and DNA nicking assay. Both sulfated polysaccharides exhibited good antioxidant activities. The sulfated polysaccharides showed strong angiotensin I-converting enzyme inhibitory activities (IC50 GTSP = 0.16 mg mL−1 and IC50 SHSP = 0.18 mg mL−1). These results revealed that GTSP and SHSP exhibited significant antihypertensive activities. Overall, the results indicated that grey triggerfish and smooth hound skins can be used to generate high value-added products, thus offering a valuable source of bioactive sulfated polysaccharides for application in wide range of biotechnological applications.

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Acknowledgments

This work was funded by Ministry of Higher Education and Scientific Research, Tunisia.

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Authors and Affiliations

  1. Unité Enzymes et Bioconversion, Ecole Nationale d’Ingénieurs de Sfax, Université de Sfax, 3038, Sfax, Tunisia

    Fatma Krichen, Wafa Karaoud, Nadhem Sayari, Assaâd Sila, Fatma Kallel, Semia Ellouz-Chaabouni & Ali Bougatef

  2. Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Equipe ProBioGEM, Université Lille 1, Lille, France

    Assaâd Sila

  3. Unité de Service Commun Bioréacteur Couplé à un Ultrafiltre, Ecole Nationale d’Ingénieurs de Sfax, Université de Sfax, Sfax, Tunisia

    Semia Ellouz-Chaabouni

  4. Département de Technologies Alimentaires, Institut Supérieur de Biotechnologie de Sfax, PB 1175, 3038, Sfax, Tunisia

    Ali Bougatef

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  1. Fatma Krichen
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  2. Wafa Karaoud
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  4. Assaâd Sila
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  6. Semia Ellouz-Chaabouni
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  7. Ali Bougatef
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Correspondence to Ali Bougatef.

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Krichen, F., Karaoud, W., Sayari, N. et al. Sulfated Polysaccharides from Tunisian Fish Skins: Antioxidant, DNA Damage Protective Effect and Antihypertensive Activities. J Polym Environ 24, 166–175 (2016). https://doi.org/10.1007/s10924-016-0759-6

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  • DOI: https://doi.org/10.1007/s10924-016-0759-6

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