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Excito-Repellent Responses between Culex quinquefasciatus Permethrin Susceptible and Resistant Mosquitoes

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Abstract

The behavioral responses exhibited between four permethrin-resistant field-derived populations and a long-established, permethrin susceptible colony population of Culex quinquefasciatus mosquitoes was assessed for behavioral responses to permethrin in an excito-repellency (ER) test system. After a 30-min exposure to two different insecticide concentrations (200 and 500 mg/m2) in paired contact and noncontact test designs, the initial knockdown and escape (exit) responses were recorded followed by mortality after 24-h. All five test populations rapidly escaped from the chambers treated with permethrin regardless of background insecticide susceptibility status or chemical concentration. The greatest contact escape response was seen from the colonized population with 88 % of specimens escaping the chamber within 30-min. Contact irritancy was the predominant response compared to relatively weak noncontact spatial repellency in all test populations at both low and high concentrations. At the lower concentration, the contact escape responses did not appear influenced by background susceptibility status, whereas at the higher concentration the permethrin-resistant field populations exhibited significantly reduced avoidance behavior compared to the laboratory susceptible strain.

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Acknowledgments

We thank the following institutions for financial support for this research: the Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University under the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, Ministry of Education, Thailand and The Thailand Research Fund, Senior Research Scholar Program (RTA5580002) and International Research Network (IRN58W0003).

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Authors and Affiliations

  1. Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand

    Wasana Boonyuan, Michael J. Bangs & Theeraphap Chareonviriyaphap

  2. Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Thailand CASAF, NRU-KU, Bangkok, 10900, Thailand

    Wasana Boonyuan

  3. Public Health & Malaria Control Department, International SOS, Jl. Kertajasa, Kuala Kencana, Papua, 99920, Indonesia

    Michael J. Bangs

  4. Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Galvin Life Science, Notre Dame, IN, 46556, USA

    John P. Grieco

  5. Veterinary Parasitology Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand

    Sonthaya Tiawsirisup

  6. Division of Computer and Statistics, Faculty of Liberal Art and Science, Kasetsart University, Kamphaensean, Nakhon Pathom, 73140, Thailand

    Atchariya Prabaripai

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  1. Wasana Boonyuan
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Correspondence to Theeraphap Chareonviriyaphap.

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Boonyuan, W., Bangs, M.J., Grieco, J.P. et al. Excito-Repellent Responses between Culex quinquefasciatus Permethrin Susceptible and Resistant Mosquitoes. J Insect Behav 29, 415–431 (2016). https://doi.org/10.1007/s10905-016-9570-4

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