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Novel Spectrofluorimetric Method for the Determination of Perindopril Erbumine Based on Fluorescence Quenching of Rhodamine B

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Abstract

A novel, simple and specific spectrofluorimetric method was developed and validated for the determination of perindopril erbumine (PDE). The method is based on the fluorescence quenching of Rhodamine B upon adding perindopril erbumine. The quenched fluorescence was monitored at 578 nm after excitation at 500 nm. The optimization of the reaction conditions such as the solvent, reagent concentration, and reaction time were investigated. Under the optimum conditions, the fluorescence quenching was linear over a concentration range of 1.0–6.0 μg/mL. The proposed method was fully validated and successfully applied to the analysis of perindopril erbumine in pure form and tablets. Statistical comparison of the results obtained by the developed and reference methods revealed no significant differences between the methods compared in terms of accuracy and precision. The method was shown to be highly specific in the presence of indapamide, a diuretic that is commonly combined with perindopril erbumine. The mechanism of rhodamine B quenching was also discussed.

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Conflict of Interest

The authors declare that they have no conflict of interest.

Authors' Contributions

Hanan Fael has performed the experimental and analytical work and prepared the draft of the manuscript. The supervision of this work was provided by Prof. Amir Al-Haj Sakur. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. Faculty of Pharmacy, Department of Analytical and Food Chemistry, University of Aleppo, Aleppo, Syrian Arab Republic

    Hanan Fael & Amir Al-Haj Sakur

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  1. Hanan Fael
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  2. Amir Al-Haj Sakur
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Correspondence to Hanan Fael.

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Fael, H., Sakur, A.AH. Novel Spectrofluorimetric Method for the Determination of Perindopril Erbumine Based on Fluorescence Quenching of Rhodamine B. J Fluoresc 25, 1577–1584 (2015). https://doi.org/10.1007/s10895-015-1666-2

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  • DOI: https://doi.org/10.1007/s10895-015-1666-2

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