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DNA Interaction, Photocleavage and Topoisomerase I Inhibition by Ru(II) Complex with a New Ligand Possessing Phenazine Unit

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Abstract

A new ruthenium complex with a dppz-like ligand pyidppz, [Ru(bpy)2(pyidppz)]2+ (pyidppz = 2-(pyridine-2-yl)imidazo-[4,5-b]dipyrido-[3,2-a:2′,3′-c]phenazine) has been synthesized and characterized by ES-MS, elemental analysis, 1H NMR. Intercalative mode of the complex bound to calf thymus DNA has been supported by different spectroscopic methods and viscosity measurements. The introduction of phenazine unit may be one of the main reasons for the weak emission of Ru(II) complex in aqueous solution. Under irradiation, this complex can efficiently cleave DNA. And the photocleavage reaction of the complex is found to be inhibited in the presence of singlet oxygen scavenger. Topoisomerase inhibition and DNA strand passage assay demonstrated that [Ru(bpy)2(pyidppz)]2+ and its parent complex [Ru(bpy)2(pyip)]2+ (pyip = 2-(pyridine-2-yl)imidazo[4,5-f][1,10]phenanthroline) can act as efficient catalytic inhibitor of DNA topoisomerase I.

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Acknowledgments

We are grateful to the supports of the National Natural Science Foundation of China (21205039, 21342012), the Natural Science Foundation of Hunan Province (13JJ6071, 14JJ7073), the experiment project of Undergraduate Innovative Training Center in Hunan province (Materials Science and Engineering) (15YCZD02) and the construct program of the key discipline in Hunan province (Applied Chemistry).

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  1. College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, ChangDe, 415000, People’s Republic of China

    Xue-Wen Liu, You-Ming Shen, Jun-Shi Shu, Yang Xiao, Song-Bai Zhang & Ji-Lin Lu

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  1. Xue-Wen Liu
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Correspondence to Xue-Wen Liu or You-Ming Shen.

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Liu, XW., Shen, YM., Shu, JS. et al. DNA Interaction, Photocleavage and Topoisomerase I Inhibition by Ru(II) Complex with a New Ligand Possessing Phenazine Unit. J Fluoresc 25, 1527–1535 (2015). https://doi.org/10.1007/s10895-015-1644-8

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