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Energy Transfer Studies between Trp Residues of Three Lipocalin Proteins Family, α1-Acid Glycoprotein, (Orosomucoid), β-Lactoglobulin and Porcine Odorant Binding Protein and the Fluorescent Probe, 1-Aminoanthracene (1-AMA)

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Abstract

Energy transfer studies between Trp residues of α1-acid glycoprotein, β-lactoglobulin and porcine odorant binding protein (OBP) and the fluorescent probe 1-aminoanthracene (1-AMA) were performed. 1-AMA binds to the hydrophobic binding sites of the three proteins inducing a decrease in the fluorescence intensity of the Trp residues accompanied by an increase of that of 1-AMA. Our results indicate that 1-AMA is in close contact with hydrophobic tryptophan residue of β-lactoglobulin (Trp 19) to the difference of its binding to OBP, where Trp residues are far from the pocket and to α1-acid glycoprotein where three Trp residues are present at different areas of the protein.

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Authors and Affiliations

  1. Laboratoire de Biophysique Moléculaire, Université Lille Nord de France, Université de Lille 1, Bâtiment C6, 59655, Villeneuve d’Ascq, France

    Jihad R. Albani, Loïc Bretesche, Julie Vogelaer & Daniel Kmiecik

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  1. Jihad R. Albani
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  2. Loïc Bretesche
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  3. Julie Vogelaer
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  4. Daniel Kmiecik
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Correspondence to Jihad R. Albani.

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Albani, J.R., Bretesche, L., Vogelaer, J. et al. Energy Transfer Studies between Trp Residues of Three Lipocalin Proteins Family, α1-Acid Glycoprotein, (Orosomucoid), β-Lactoglobulin and Porcine Odorant Binding Protein and the Fluorescent Probe, 1-Aminoanthracene (1-AMA). J Fluoresc 25, 167–172 (2015). https://doi.org/10.1007/s10895-014-1493-x

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