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Fluorescence Determination of Azithromycin in Pharmaceutical Formulations by Using the Synchronous Scanning Approach After its Acid Derivatization

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Abstract

In this present work, a fluorescence method for azithromycin (9-deoxo-9a-aza-9a-methyl-9a-homoerythromycin) determination in pharmaceutical formulations is proposed. The method is based on the synchronous fluorescence (Δλ = 30 nm, 482 nm) produced when azithromycin is derivatized in strong acidic medium (9.0 mol L−1 HCl). The influence of the derivatization conditions (acid concentration, reaction time and temperature) was studied. Also, the possible reaction mechanism was discussed. In the optimized conditions, the method presented a limit of detection of 0.23 mg L−1 and a limit of quantification of 0.76 mg L−1. The developed procedure was successfully applied in the determination of azithromycin in pharmaceutical formulations.

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Acknowledgments

The authors are grateful to CNPq, CAPES and FAPERJ for the grants, fellowships and scholarships and for the financial support.

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Authors and Affiliations

  1. Departamento de Química Analítica, Universidade Federal Fluminense, Outeiro de São João Batista s/n, Centro, Niterói, RJ, 24020-141, Brazil

    Vanessa G. K. Almeida, Victor S. M. Braga, Wagner F. Pacheco & Ricardo J. Cassella

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  1. Vanessa G. K. Almeida
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  2. Victor S. M. Braga
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  3. Wagner F. Pacheco
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  4. Ricardo J. Cassella
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Correspondence to Ricardo J. Cassella.

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Almeida, V.G.K., Braga, V.S.M., Pacheco, W.F. et al. Fluorescence Determination of Azithromycin in Pharmaceutical Formulations by Using the Synchronous Scanning Approach After its Acid Derivatization. J Fluoresc 23, 31–39 (2013). https://doi.org/10.1007/s10895-012-1111-8

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  • DOI: https://doi.org/10.1007/s10895-012-1111-8

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