Journal of Fluorescence

, Volume 21, Issue 1, pp 409–414

Study on the Interaction of an Anthracycline Disaccharide with DNA by Spectroscopic Techniques and Molecular Modeling

Authors

    • School of Chemistry and Environmental ScienceHenan Normal University
  • Gong-Ke Wang
    • School of Chemistry and Environmental ScienceHenan Normal University
  • Juan Lv
    • School of Chemistry and Environmental ScienceHenan Normal University
  • Gui-Sheng Zhang
    • School of Chemistry and Environmental ScienceHenan Normal University
  • Qing-Feng Liu
    • School of Chemistry and Environmental ScienceHenan Normal University
Original Paper

DOI: 10.1007/s10895-010-0729-7

Cite this article as:
Lu, Y., Wang, G., Lv, J. et al. J Fluoresc (2011) 21: 409. doi:10.1007/s10895-010-0729-7

Abstract

This study was designed to examine the interaction of 4′-O-(a-L-Cladinosyl) daunorubicin (DNR–D5), a disaccharide anthracycline with calf thymus deoxyribonucleic acid (ctDNA) by UV/Vis in combination with fluorescence spectroscopy and molecular modeling techniques under physiological conditions (Britton–Robinson buffer solutions, pH = 7.4). By the analysis of UV/Vis spectrum, it was observed that upon binding to ctDNA the anthraquinone chromophore of DNR–D5 could slide into the base pairs. Moreover, the large binding constant indicated DNR–D5 had a high affinity with ctDNA. At the same time, fluorescence spectra suggested that the quenching mechanism of the interaction of DNR–D5 to ctDNA was a static quenching type. The binding constants between DNR–D5 and ctDNA were calculated based on fluorescence quenching data at different temperatures. The negative ∆G implied that the binding process was spontaneous, and negative ∆H and negative ΔS suggested that hydrogen bonding force most likely played a major role in the binding of DNR–D5 to ctDNA. Moreover, the results obtained from molecular docking corroborate the experimental results obtained from spectroscopic investigations.

Keywords

DNR–D5ctDNAFluorescence spectroscopyUV/VisMolecular modeling

Copyright information

© Springer Science+Business Media, LLC 2010