Male-Specific Cuticular Compounds of the Six Drosophila paulistorum Semispecies: Structural Identification and Mating Effect
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
We have identified cuticular pheromones that sustain the integrity of the six Drosophila paulistorum semispecies. Hexane extracts of male and female cuticles were separated on a silica gel column and analyzed using gas chromatography/mass spectrometry. Both sexes of each of the six semispecies have the same fifteen major cuticular components, all hydrocarbons ranging from C31 to C37. However, all males have four additional ester compounds. Bioassay observations showed that this four-component ester complex imparts a strong anti-aphrodisiac effect on intra-semispecific mating behavior, thus confirming its pheromonal role. The three major ester components are methyl (Z)-9-tetradecenoate (C15H28O2), 11-docosenyl acetate (C24H46O2), and 19-triacontenyl acetate (C32H62O2). The fourth ester is a di-unsaturated acetate with molecular formula C32H60O2, but the positions of unsaturation have not been determined. Bioassays indicate that the male-specific complex of the transitional semispecies, the relict ancestor, imparts anti-aphrodisiac effects on the other semispecies as well, but effectiveness decreases with phylogenetic distances. Across the six semispecies, the male-specific compounds are the same, but vary quantitatively. Apparently, the quantitative differences among these incipient species act efficiently to preclude hybridization in nature. Because Drosophila paulistorum is a cluster of incipient species, this opportunity to observe pheromonal influences on speciation is unique.
- Buser, H-R., Arn, H., Guerin, P., and Rauscher, S. 1983. Determination of double bond position in mono-unsaturated acetates by mass spectrometry of dimethyl disulfide adducts. Anal. Chem. 55:818–822. CrossRef
- Carlson, D.A., Roan, C-S., Yost, R.A. and Hector, J. 1989. Dimethyl disulfide derivatives of long chain alkenes, alkadienes, and alkatrienes for gas chromatography/mass spectrometry. Anal. Chem., 61, 1564–1571. CrossRef
- Chao, T.H., Gheta, A., Kim, Y-K., Cella, R., and Ehrman, L. 2001. Male pheromones of Drosophila paulistorum. Drosophila Information Service, 84:50–54.
- Dobzhansky,T and Spassky, B. 1959. Drosophila paulistorum, a cluster of species in Statu Nascendi. Proc. Natl.Acad.Sci.USA, 45:419–428. CrossRef
- Ehrman, L. 1965. Direct observation of sexual isolation between allopatric and between sympatric strains of the different Drosophila paulistorum races. Evolution 19:459–464. CrossRef
- Ehrman, L. 1975. Reproductive Isolation pp. 71–74 in Drosophila. Animal Behavior in Laboratory and Field. E.O. Price (ed.). Second ed. rev. San Francisco: Wm Freeman, and Teachers Manual, 35–36.
- Ehrman, L. and Kim, Y-K. 1998. Courtship. pp.638-648 in Encyclopedia of Comparative Psychology. G. Greenberg and M. Haraway (eds.). Garland: New York.
- Ehrman, L. and Powell, J. 1982. The Drosophila willistoni species group. pp. 193-225 in The Genetics and Biology of Drosophila. M. Ashburner, H.L. Carson and J.N. Thompson (eds.). London:Academic,.
- Ehrman L., Perelle, I., and Factor, J. 1995. Endosymbiotic infectivity in Drosophila paulistorum semispecies. Chapter 18, 241–261 in L. Levine (ed.) Genetics of Natural Populations, The Continuing Influence of Theodosius Dobzhansky. Columbia University Press: New York.
- Elens, A.A., and Wattiaux J. M. 1964. Direct observation of sexual isolation. Drosophila Information Service 39:118–119.
- Ferveur, J. 1996. The pheromonal role of cuticular hydrocarbons in Drosophila melanogaster. BioEssays 19:353–358. CrossRef
- Gleason, J.M., Griffith, E.C., and Powell, J.R. 1998. A molecular phylogeny of the Drosophila willistoni group: conflicts between species, Evolution 52:1093–1103. CrossRef
- Harrison, A.G. 1992 Chemical Ionization Mass Spectrometry, 2nd ed. CRC Press, p. 131–134.
- Jallon, J.-M. 1984 A few chemical words exchanged by Drosophila during courtship and mating. Behav. Genet. 14: 441–476. CrossRef
- Jallon, J.-M. and Penchine, J.M. 1989. Une autre race chimique de Drosophila melanogaster en Afrique. C.R. Acad. Sci. Paris 309: 1551–1556.
- Kim, Y-K, Phillips, D.R., Chao, T., and Ehrman, L. 2004. Developmental isolation and subsequent adult behavior of Drosophila paulistorum. VI. Quantitative variation in cuticular hydrocarbons” Behav. Genet. 34: 385–394. CrossRef
- Knoppien, P. 1985. Rare-male advantage: a review. Biological Reviews 60:81–117. CrossRef
- Leonard, J. and Ehrman, L. 1983. Does the rare male advantage result from faulty experimental design? Genetics 104:713–716.
- Marcillac, F., Grosjean, Y., and Ferveur, J.-F. 2005. A single mutation alters production and discrimination of Drosophila sex pheromones. Proc. R. Soc. B 272:303–309. CrossRef
- Mclafferty, F.W. and Turecek, F. 1993. Interpretation of Mass Spectra 4th ed. pp. 252–257.
- Richmond, R. and Ehrman, L. 1974. Incidence of repeated mating in Drosophila paulistorum. Experientia 30:489–490. CrossRef
- Male-Specific Cuticular Compounds of the Six Drosophila paulistorum Semispecies: Structural Identification and Mating Effect
Journal of Chemical Ecology
Volume 36, Issue 9 , pp 933-942
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Drosophila paulistorum
- Male pheromone
- Cuticular hydrocarbon
- Methyl (Z)-9-tetradecenoate
- 11-docosenyl acetate
- 19-triacontenyl acetate
- Industry Sectors