Abstract
We report that the bacterium Azospirillum brasilense can synthesize silver and gold nanoparticles. Discrete spherical nanoparticles of silver and colloidal gold of diverse shapes were found to accumulate in the culture liquid of A. brasilense strain Sp245. We show for the first time that bacterial extracellular Mn-peroxidase is involved in the reduction of silver from silver nitrate and in the reduction of gold from chloroauric acid to a nanoparticle form. The enzymatically fabricated particles were characterized by UV spectroscopy, atomic force and transmission electron microscopy, X-ray fluorescence, and dynamic light scattering. A mechanism is proposed for the Mn-peroxidase-aided biogenesis of metal nanoparticles.
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Acknowledgements
This work was supported by the Russian Foundation for Basic Research [grant number 16-34-01200 mol_a]. We thank Dmitry N. Tychinin (this institute) for the English version of this manuscript.
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Kupryashina, M.A., Vetchinkina, E.P. & Nikitina, V.E. Biofabrication of Discrete Silver and Gold Nanoparticles by the Bacterium Azospirillum brasilense: Mechanistic Aspects. J Clust Sci 28, 1179–1190 (2017). https://doi.org/10.1007/s10876-016-1111-y
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DOI: https://doi.org/10.1007/s10876-016-1111-y