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Water Soluble Melanin of Streptomyces lusitanus DMZ3 Persuade Synthesis of Enhanced Bio-medically Active Silver Nanoparticles

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Abstract

Enhanced bio-medically active silver nanoparticles were produced from water soluble melanin of Streptomyces lusitanus DMZ3. Synthesis of silver nanoparticles within 80 s was facilitated by microwave radiation. Melanin silver nanoparticles produced in the range of 13–22 nm sizes were confirmed and characterized by UV–Vis spectrum, transmission electron microscopy, dynamic light scattering, X-ray diffraction and Fourier transform infrared spectroscopy analysis. Silver nanoparticles produced from water soluble melanin showed potential biomedical attributes. Promising antioxidant activity of melanin silver nanoparticles with 130 µg/mL IC50 (50 % inhibition concentration) and 82.5 µg/mL EC50 (50 % effective concentration) functional potentials were recorded following DPPH (2,2′diphenyl-1-picryldrazyl) method and phosphomolybdate assay respectively. These melanin silver nanoparticles were found to be potential cytotoxic agent with 0.34 µg/mL LC50 (50 % lethal concentration) employing brine shrimps. Water soluble melanin, explored in the study, naturally would be a strategic biological reducing agent to produce melanin silver nanoparticles with greater biomedical activities.

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Acknowledgments

The Grant to carry out the present research work provided by the Department of Biotechnology (BT/PR13864/PID/06/580/2010, Dt. 05.08.2011), Government of India, New Delhi is sincerely acknowledged.

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Authors and Affiliations

  1. A-DBT Research Laboratory, Department of Microbiology, Gulbarga University, Gulbarga, 585 106, India

    D. N. Madhusudhan & Dayanand Agsar

  2. Department of Biotechnology, Gulbarga University, Gulbarga, 585 106, India

    M. B. Sulochana

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  1. D. N. Madhusudhan
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  2. Dayanand Agsar
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  3. M. B. Sulochana
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Correspondence to Dayanand Agsar.

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Madhusudhan, D.N., Agsar, D. & Sulochana, M.B. Water Soluble Melanin of Streptomyces lusitanus DMZ3 Persuade Synthesis of Enhanced Bio-medically Active Silver Nanoparticles. J Clust Sci 26, 1077–1089 (2015). https://doi.org/10.1007/s10876-014-0798-x

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