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Kinetics of Effector Functions and Phenotype of Virus-Specific and γδ T Lymphocytes in Primary Human Cytomegalovirus Infection During Pregnancy

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Abstract

The T-cell response to human cytomegalovirus (HCMV) primary infection was analyzed in 27 pregnant women during the first year after primary HCMV infection. Pregnant women with remote HCMV infection were enrolled as controls. Interferon-γ-producing T cells were readily detected at levels comparable (CD4+) or higher (CD8+) than controls, whereas the CD4+ and CD8+ lymphoproliferative response as well as IL-2 production was significantly reduced with respect to controls for at least 9 months after infection. In addition, CD45RA re-expression as well as cytotoxic T lymphocyte activity and perforin expression were the major components of the adaptive CD4+ and CD8+ T-cell immune response, while Vδ2 γδ T-cell expansion in response to HCMV infection followed kinetics similar to that of CD8+ T cells. Reduced CD45RA re-expression directly correlated with HCMV transmission to the fetus, thus providing an important prognostic parameter.

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Acknowledgments

The entire technical staff of the Virology Unit is gratefully acknowledged. In addition, we are indebted to Daniela Sartori for careful preparation of the manuscript and to Laurene Kelly for competent revision of the English. This work was partially supported by the Ministero della Salute, Fondazione IRCCS Policlinico San Matteo, Ricerca Corrente (grants 80425 and 80513) and Ricerca Finalizzata (grant 89301), and by Fondazione Carlo Denegri (FCD), Torino, Italy.

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Correspondence to Giuseppe Gerna.

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Fornara, C., Lilleri, D., Revello, M.G. et al. Kinetics of Effector Functions and Phenotype of Virus-Specific and γδ T Lymphocytes in Primary Human Cytomegalovirus Infection During Pregnancy. J Clin Immunol 31, 1054–1064 (2011). https://doi.org/10.1007/s10875-011-9577-8

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