Article

Journal of Clinical Immunology

, Volume 31, Issue 6, pp 1054-1064

Kinetics of Effector Functions and Phenotype of Virus-Specific and γδ T Lymphocytes in Primary Human Cytomegalovirus Infection During Pregnancy

  • Chiara FornaraAffiliated withExperimental Research Laboratories, Transplantation Area, Fondazione IRCCS Policlinico San MatteoExperimental Research Laboratories, Biotechnology Area, Fondazione IRCCS Policlinico San Matteo
  • , Daniele LilleriAffiliated withExperimental Research Laboratories, Transplantation Area, Fondazione IRCCS Policlinico San Matteo
  • , M. Grazia RevelloAffiliated withDepartment of Obstetrics and Gynaecology, Fondazione IRCCS Policlinico San Matteo
  • , Milena FurioneAffiliated withMolecular Virology Unit, Virology and Microbiology Service, Fondazione IRCCS Policlinico San Matteo
  • , Maurizio ZavattoniAffiliated withMolecular Virology Unit, Virology and Microbiology Service, Fondazione IRCCS Policlinico San Matteo
  • , Elisa LentaAffiliated withMolecular Virology Unit, Virology and Microbiology Service, Fondazione IRCCS Policlinico San Matteo
  • , Giuseppe GernaAffiliated withExperimental Research Laboratories, Transplantation Area, Fondazione IRCCS Policlinico San Matteo Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

The T-cell response to human cytomegalovirus (HCMV) primary infection was analyzed in 27 pregnant women during the first year after primary HCMV infection. Pregnant women with remote HCMV infection were enrolled as controls. Interferon-γ-producing T cells were readily detected at levels comparable (CD4+) or higher (CD8+) than controls, whereas the CD4+ and CD8+ lymphoproliferative response as well as IL-2 production was significantly reduced with respect to controls for at least 9 months after infection. In addition, CD45RA re-expression as well as cytotoxic T lymphocyte activity and perforin expression were the major components of the adaptive CD4+ and CD8+ T-cell immune response, while Vδ2 γδ T-cell expansion in response to HCMV infection followed kinetics similar to that of CD8+ T cells. Reduced CD45RA re-expression directly correlated with HCMV transmission to the fetus, thus providing an important prognostic parameter.

Keywords

T-cell immune response γδ T cells primary HCMV infection cytotoxicity perforin