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Concentration-dependent effects on fully hydrated DNA at terahertz frequencies

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Abstract

Using terahertz time-domain spectroscopy (THz-TDS), the frequency-dependent dielectric constant of deoxyribonucleic acid (DNA) in solution was measured. The response of the buffer solution is dominated by two Debye modes in this frequency range, and, from an analysis of the concentration dependence, the presence of the DNA increases the main relaxation time and dielectric constant. This reflects the fact that the water in the hydration layer is more tightly bound under the influence of the DNA molecule in comparison to bulk water. This dynamical slowing down with increasing DNA concentration is similar to what is observed with purine nucleotides, but opposite to the behavior of pyrimidine nucleotides. In addition, a suspension model was used with the concentration-dependent data to isolate the dielectric response of the hydrated DNA molecule. The data for the hydrated DNA molecule is still dominated by a Debye response. It is also possible to determine the thickness of the hydration layer, and the DNA molecule influences the surrounding water out to 16 or 17 Å, which corresponds to about six effective hydration layers.

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Acknowledgments

This work was funded by the Cooperative Agreement on Research and Education (CARE) program with Los Alamos National Laboratory and the advisement and laboratory of W.P. Beyermann.

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  1. Department of Physics and Astronomy, University of California, Riverside, Riverside, CA, 92521, USA

    P. Glancy

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Glancy, P. Concentration-dependent effects on fully hydrated DNA at terahertz frequencies. J Biol Phys 41, 247–256 (2015). https://doi.org/10.1007/s10867-015-9377-0

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  • DOI: https://doi.org/10.1007/s10867-015-9377-0

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