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Chemical modifications of respiratory complex I for structural and functional studies

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Abstract

Studies on chemical modifications of bacterial and mitochondrial complex I by synthetic chemical probes as well as endogenous chemicals have provided useful information on the structural and functional aspects of this enzyme. We herein reviewed recent studies that investigated chemical modifications of complex I by endogenous chemicals (e.g. Cys-S-nitrosation, Cys-S-glutathionylation, and Ser-O-phosphorylation) and synthetic reagents (e.g. Cys-SH modification by SH-reagents and the cross-linking of nearby subunits by bifunctional cross-linkers). We also reviewed recent photoaffinity labeling studies using complex I inhibitors, which can be recognized as “site-specific modification” by synthetic chemicals. In addition, we discussed the possibility of site-specific modification by various functional probes via ligand-directed tosylate (LDT) chemistry as a promising approach for unique biophysical studies on complex I.

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Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research (Grant 23380064 to H. M.) and for Young Scientists (Grant 23780116 to M. M.) from the Japan Society for the Promotion of Science.

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The authors declare that they have no conflict of interest.

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  1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan

    Masatoshi Murai & Hideto Miyoshi

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  1. Masatoshi Murai
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Correspondence to Hideto Miyoshi.

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Murai, M., Miyoshi, H. Chemical modifications of respiratory complex I for structural and functional studies. J Bioenerg Biomembr 46, 313–321 (2014). https://doi.org/10.1007/s10863-014-9562-z

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