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13C- and 1H-detection under fast MAS for the study of poorly available proteins: application to sub-milligram quantities of a 7 trans-membrane protein

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Abstract

We demonstrate that 13C-detected spectra recorded using fast (60 kHz) magic angle spinning on sub-milligram (<10 μmol) quantities of a protonated 7 trans-membrane helix protein (bacteriorhodopsin) in its native lipid environment are comparable in sensitivity and resolution to those recorded using 15-fold larger sample volumes with conventional solid state NMR methodology. We demonstrate the utility of proton-detected measurements which yield narrow 1H linewidths under these conditions, and that no structural alterations are observed. We propose that these methods will prove useful to gain structural information on membrane proteins with poor availability, which can be studied in their native lipid environments.

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Acknowledgments

We gratefully acknowledge funding from the Medical Research Council (MRC), the Biotechnology and Biological Sciences Research Council (BBSRC), and the Engineering and Physical Sciences Research Council (EPSRC). We acknowledge the Bio-NMR initiative (EC-FP7 Project Number: 261863) for funding access to the CRMN facility in Lyon where all 60 kHz MAS data was recorded, and we thank Moreno Lelli for his assistance during these visits. We acknowledge the use of the solid state NMR facility at the University of Warwick where preliminary fast spinning NMR data was recorded.

Conflict of interest

The authors declare no conflict of interest.

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    Authors and Affiliations

    1. Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK

      Hugh R. W. Dannatt, Garrick F. Taylor, Krisztina Varga, Victoria A. Higman, Marc-Philipp Pfeil, Lubica Asilmovska, Peter J. Judge & Anthony Watts

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    1. Hugh R. W. Dannatt
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    2. Garrick F. Taylor
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    3. Krisztina Varga
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    4. Victoria A. Higman
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    Correspondence to Anthony Watts.

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    Hugh R. W. Dannatt and Garrick F. Taylor have contributed equally to this work.

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    Dannatt, H.R.W., Taylor, G.F., Varga, K. et al. 13C- and 1H-detection under fast MAS for the study of poorly available proteins: application to sub-milligram quantities of a 7 trans-membrane protein. J Biomol NMR 62, 17–23 (2015). https://doi.org/10.1007/s10858-015-9911-1

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