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Ocular biocompatibility of polyquaternium 10 gel: functional and morphological results

  • Biocompatibility Studies
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Abstract

This paper deals with the characterization study of topical and intraocular biocompatibility and toxicity of cationic hydroxyethylcellulose Polyquaternium 10 (PQ10). It also evaluates the rheological properties of gels. The cytotoxicity assays were done in two cell lines: HEp-2 and VERO (human larynx epidermoid carcinoma cell and African green monkey kidney cells respectively). For the in vivo study, New Zealand albino rabbits were used. The in vitro cytotoxic activity of PQ10 shows no statistically significant differences in relation to the control of hydroxypropylmethylcellulose (HPMC) in any of the cell lines used in this study. Similarly, the signs of inflammation observed after treatment showed no significant difference between the groups of animals treated with the polymer compared to the control group. Normal histological characteristics were seen in both groups with no histological inflammatory reaction. After 1 month of the intracameral application of 2 % PQ10 (treatment group) or 0.3 % HPMC (control group), electroretinograms showed similar levels of a- and b-waves latencies and amplitude. In summary, PQ10 gel was well tolerated in these experiments, with proper monitoring, it could stand as a new alternative in the development of ophthalmic viscosurgical devices.

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Acknowledgments

Authors thank to Dr. Karina Bierbrauer for technical assistance in rheological studies. Financial support for this study has provided from CEPROCOR and Fundación VER.

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Authors and Affiliations

  1. Centro de Excelencia en Productos y Procesos de Córdoba (CEPROCOR), Ministerio de Ciencia y Tecnología de Córdoba, Pabellón CEPROCOR, CP 5164, Santa María de Punilla, Córdoba, Argentina

    Roxana Valeria Alasino & Dante Miguel Beltramo

  2. Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina

    Roxana Valeria Alasino & Dante Miguel Beltramo

  3. Departamento de Oftalmología, Centro Privado de Ojos Romagosa-Fundación VER, Córdoba, Argentina

    Luciana Guadalupe Garcia, Ana Laura Gramajo, Juan Pablo Pusterla & José Domingo Luna

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  1. Roxana Valeria Alasino
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  2. Luciana Guadalupe Garcia
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  3. Ana Laura Gramajo
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  4. Juan Pablo Pusterla
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  5. Dante Miguel Beltramo
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Correspondence to Dante Miguel Beltramo or José Domingo Luna.

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Alasino, R.V., Garcia, L.G., Gramajo, A.L. et al. Ocular biocompatibility of polyquaternium 10 gel: functional and morphological results. J Mater Sci: Mater Med 26, 64 (2015). https://doi.org/10.1007/s10856-014-5358-2

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  • DOI: https://doi.org/10.1007/s10856-014-5358-2

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