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Long-term biocompatibility evaluation of 0.5 % zinc containing hydroxyapatite in rabbits

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Abstract

This study investigates the long-term biocompatibility of 0.5 % zinc-containing hydroxyapatite compared with hydroxyapatite. Spheres (425 < ∅ < 550) of both materials were produced by extrusion of ceramic slurry in calcium chloride and characterized by FTIR, XRD, XRF and SEM. Fifteen White New Zealand rabbits were submitted to general anesthesia, and an perforation (2 mm), was made in each tibia, one for zinc-containing hydroxyapatite sphere implantation and one for hydroxyapatite sphere implantation. After 26, 52 and 78 weeks, the animals were euthanized, and the fragment containing the biomaterial was harvested. A 30–50 μm section was obtained for histological analysis in bright field and polarized light. SEM images revealed similar morphologies between the tested biomaterials. Histological analysis showed that there was no difference between the test groups. The morphometric analysis, however, indicates that there was a greater absorption. The materials are biocompatible, promote osteogenesis and that the zinc-containing hydroxyapatite microspheres were absorbed more quickly.

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Acknowledgments

The authors acknowledge the financial support of FAPERJ, FINEP, DECIT-MS and CNPq. In addition to the research partners LNLS, COPPE, INMETRO and CBPF.

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Authors and Affiliations

  1. Cell and Molecular Biology Department, Biology Institute, Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil

    Rodrigo F. B. Resende, Gustavo V. O. Fernandes & José M. Granjeiro

  2. Biomaterials Laboratory-LABIOMAT, Brazilian Physics of Center Researches, Rio de Janeiro, Brazil

    Sílvia R. A. Santos & Alexandre M. Rossi

  3. Nuclear Instrumentation Laboratory, Nuclear Engineering Program, COPPE/UFRJ, Rio de Janeiro, Brazil

    Inayá Lima

  4. Bioengineering Department, National Institute of Metrology, Quality and Technology, Duque de Caxias, Rio de Janeiro, Brazil

    José M. Granjeiro

  5. Clinical Research Unit, Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil

    José M. Granjeiro

  6. Oral Surgery Department—Dentistry School, Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil

    Rodrigo F. B. Resende & Mônica D. Calasans-Maia

Authors
  1. Rodrigo F. B. Resende
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  2. Gustavo V. O. Fernandes
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  3. Sílvia R. A. Santos
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  4. Alexandre M. Rossi
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  5. Inayá Lima
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  6. José M. Granjeiro
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  7. Mônica D. Calasans-Maia
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Correspondence to Rodrigo F. B. Resende.

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Resende, R.F.B., Fernandes, G.V.O., Santos, S.R.A. et al. Long-term biocompatibility evaluation of 0.5 % zinc containing hydroxyapatite in rabbits. J Mater Sci: Mater Med 24, 1455–1463 (2013). https://doi.org/10.1007/s10856-013-4865-x

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  • DOI: https://doi.org/10.1007/s10856-013-4865-x

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