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Comparative study of three types of polymer materials co-cultured with bone marrow mesenchymal stem cells for use as a myocardial patch in cardiomyocyte regeneration

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Abstract

The purpose of this study was to investigate the most suitable polymer material for supporting stem cell growth as a myocardial patch. After cell isolation and expansion of mouse bone marrow mesenchymal stem cells (BMSC), the cells were induced to differentiate into cardiomyocytes with 5-azacytidine to determine their differentiation potential. BMSCs were also seeded onto three types of polymer material film, including polyurethane (PU), 3-hydroxybutyrate-co-4-hydroxybutyrate [P(3HB-co-4HB)], and polypropylene carbonate (PPC). The results revealed that cell numbers were more abundant on both the PU and P(3HB-co-4HB) material surfaces. Conversely, the surface of PPC was smooth with only cell lysate debris observed. The average cell counts were as follows: 143.78 ± 38.38 (PU group), 159.50 ± 33.07 [P(3HB-co-4HB) group], and 1.40 ± 0.70 (PPC group). There was no statistically significant difference in cell numbers between the PU and P(3HB-co-4HB) groups. A statistically significant difference was identified between the PPC group and both the PU (P1) and P(3HB-co-4HB) groups (P2). Polymer biomaterial patches composed of PU and P(3HB-co-4HB) permit good stem cell growth. P(3HB-co-4HB) has the potential for development as a clinical alternative to current treatment methods for the regeneration of cardiomyocytes in patients with myocardial infarction.

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References

  1. Teng CJ, Luo J, Chiu RC, Shum-Tim D. Massive mechanical loss of microspheres with direct intramyocardial inject ion in the beating heart: implications for cellular cardiomyoplasty. J Thorac Cardiovasc Surg. 2006;132:628–32.

    Article  Google Scholar 

  2. Al Kindi AH, Asenjo JF, Ge Y, Chen GY, Bhathena J, Chiu RC, Prakash S, Shum-Tim D. Microencapsulation to reduce mechanical loss of microspheres: implications in myocardial cell therapy. Eur J Cardiothorac Surg. 2011;39:241–7.

    Article  Google Scholar 

  3. Saito T, Kuang JQ, Lin CC, Chiu RC. Transcoronary implantation of bone marrow stromal cells ameliorates cardiac function after myocardial infarction. J Thorac Cardiovasc Surg. 2003;126:114–23.

    Article  Google Scholar 

  4. MacDonald DJ, Luo J, Saito T, Duong M, Bernier PL, Chiu RC, Shum-Tim D. Persistence of marrow stromal cells implanted into acutely infarcted myocardium: observations in a xenotransplant model. J Thorac Cardiovasc Surg. 2005;130:1114–21.

    Article  Google Scholar 

  5. Hui J, Huishang W, Zhengwei W. The gene express of differentiation of human bone marrow mesenchymal stem cells into myocardial cell. J Cardiovasc Pulm Dis. 2010;29:43–8.

    Google Scholar 

  6. Zhou Q, Zhou JY, Zheng Z, Zhang H, Hu SS. A novel vascularized patch enhances cell survival and modifies ventricular remodeling in a rat myocardial infarction model. J Thorac Cardiovasc Surg. 2010;140:1388–96. e1–3.

    Article  Google Scholar 

  7. Valarmathi MT, Goodwin RL, Fuseler JW, Davis JM, Yost MJ, Potts JD. A 3-D cardiac muscle construct for exploring adult marrow stem cell based myocardial regeneration. Biomaterials. 2010;31:3185–200.

    Article  CAS  Google Scholar 

  8. Köse GT, Korkusuz F, Korkusuz P, Purali N, Ozkul A, Hasirci V. Bone generation on PHBV matrices: an in vitro study. Biomaterials. 2003;24:4999–5007.

    Article  Google Scholar 

  9. Shachar M, Tsur-Gang O, Dvir T, Leor J, Cohen S. The effect of immobilized RGD peptide in alginate scaffolds on cardiac tissue engineering. Acta Biomater. 2011;7:152–62.

    Article  CAS  Google Scholar 

  10. Park BW, Kang EJ, Byun JH, Son MG, Kim HJ, Hah YS, Kim TH, Mohana Kumar B, Ock SA, Rho GJ. In vitro and in vivo osteogenesis of human mesenchymal stem cells derived from skin, bone marrow and dental follicle tissues. Differentiation. 2012;83:249–59.

    Article  CAS  Google Scholar 

  11. Sapir Y, Kryukov O, Cohen S. Integration of multiple cell-matrix interactions into alginate scaffolds for promoting cardiac tissue regeneration. Biomaterials. 2011;32:1838–47.

    Article  CAS  Google Scholar 

  12. Saeed H, Taipaleenmäki H, Aldahmash AM, Abdallah BM, Kassem M. Mouse embryonic fibroblasts (MEF) exhibit a similar but not identical phenotype to bone marrow stromal stem cells (BMSC). Stem Cell Rev. 2011;7:161–71.

    Article  Google Scholar 

  13. Zhang S, Ge J, Sun A, Xu D, Qian J, Lin J, Zhao Y, Hu H, Li Y, Wang K, Zou Y. Comparison of various kinds of bone marrow stem cells for the repair of infarcted myocardium: single clonally purified non-hematopoietic mesenchymal s tem cells serve as a superior source. J Cell Biochem. 2006;99:1132–47.

    Article  CAS  Google Scholar 

  14. Jain M, Pfister O, Hajjar RJ, Liao R. Mesenchymal stem cells in the infarcted heart. Coron Artery Dis. 2005;16:93–7.

    Article  Google Scholar 

  15. Martens TP, See F, Schuster MD, Sondermeijer HP, Hefti MM, Zannettino A, Gronthos S, Seki T, Itescu S. Mesenchymal lineage precursor cells induce vascular network formation in ischemic myocardium. Nat Clin Pract Cardiovasc Med. 2006;3:S18–22.

    Article  CAS  Google Scholar 

  16. Antonitsis P, Ioannidou-Papagiannaki E, Kaidoglou A. Cardiomyogenic potential of human adult bone marrow mesenchymal stem cells in vitro. Thorac Cardiovasc Surg. 2008;56:77–82.

    Article  CAS  Google Scholar 

  17. Antonitsis P, Ioannidou-Papagiannaki E, Kaidoglou A, et al. In vitro cardiomyogenic differentiation of adult human bone marrow mesenchymal stem cells. The role of 5-azacytidine. Interact Cardiovasc Thorac Surg. 2007;6:593–7.

    Article  Google Scholar 

  18. Al Kindi AH, Asenjo JF, Ge Y, Chen GY, Bhathena J, Chiu RC, Prakash S, Shum-Tim D. Micro encapsulation to reduce mechanical loss of microspheres implications in myocardial cell therapy. Eur J Cardiothorac Surg. 2011;39:241–7.

    Article  Google Scholar 

  19. Darby RT, Kaplan AM. Fungal susceptibility of polyurethanes. Appl Microbiol. 1968;16:900–5.

    CAS  Google Scholar 

  20. Lioyd DR, Kim SS, Kinzer KE. Microporous membrane formation via thermally-induced phase separation. J Membr Sci. 1991;64:13–28.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, China

    Hongxing Niu, Junsheng Mu, Jianqun Zhang & Ping Bo

  2. Department of Chemistry, High Polymer Institute Laboratory, Tsinghua University, Beijing, 100084, China

    Ping Hu & Yan Wang

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  1. Hongxing Niu
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  2. Junsheng Mu
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Correspondence to Junsheng Mu.

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Niu, H., Mu, J., Zhang, J. et al. Comparative study of three types of polymer materials co-cultured with bone marrow mesenchymal stem cells for use as a myocardial patch in cardiomyocyte regeneration. J Mater Sci: Mater Med 24, 1535–1542 (2013). https://doi.org/10.1007/s10856-012-4842-9

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  • DOI: https://doi.org/10.1007/s10856-012-4842-9

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