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A novel cyclic RGD-containing peptide polymer improves serum-free adhesion of adipose tissue-derived mesenchymal stem cells to bone implant surfaces

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Abstract

Seeding of bone implants with mesenchymal stem cells (MSCs) may promote osseointegration and bone regeneration. However, implant material surfaces, such as titanium or bovine bone mineral, fail to support rapid and efficient attachment of MSCs, especially under serum-free conditions that may be desirable when human applications or tightly controlled experiments are envisioned. Here we demonstrate that a branched poly[Lys(Seri-DL-Alam)] polymer functionalized with cyclic arginyl-glycyl-aspartate, when immobilized by simple adsorption to tissue culture plastic, surgical titanium alloy (Ti6Al4V), or Bio-Oss® bovine bone substitute, significantly accelerates serum-free adhesion and enhances seeding efficiency of human adipose tissue-derived MSCs. Moreover, when exposed to serum-containing osteogenic medium, MSCs survived and differentiated on the peptide-coated scaffolds. In summary, the presented novel polypeptide conjugate can be conveniently used for coating various surfaces, and may find applications whenever quick and efficient seeding of MSCs is required to various scaffolds in the absence of serum.

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Acknowledgments

The authors would like to thank all colleagues at the Tissue Regeneration Department of the Twente University for the kind support, as well as Éva Juhász and Balázs Hegedűs for the help with time-lapse microscopy. This work was financially supported by the grants BIO_SURF from the National Office for Research and Technology (NKTH) and TÁMOP-4.2.1-IKUT from the National Development Agency (NFÜ).

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Authors and Affiliations

  1. Research Center for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary

    Péter Tátrai & Gergely Szakács

  2. Department of Experimental Gene Therapy, National Blood Transfusion Service, Diószegi út 64, Budapest, 1113, Hungary

    Péter Tátrai & Katalin Német

  3. Stem Cell Laboratory, National Blood Transfusion Service, Budapest, Hungary

    Bernadett Sági & Ferenc Uher

  4. Creative Cell Ltd, Budapest, Hungary

    Anna Szigeti, Áron Szepesi & Katalin Német

  5. Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Budapest, Hungary

    Ildikó Szabó, Szilvia Bősze & Gábor Mező

  6. Department of Plant Anatomy, Eötvös Loránd University, Budapest, Hungary

    Zoltán Kristóf

  7. Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary

    Károly Markó

  8. Department of Restorative Dentistry, Loma Linda University, Loma Linda, CA, USA

    István Urbán

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  1. Péter Tátrai
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Correspondence to Katalin Német.

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Tátrai, P., Sági, B., Szigeti, A. et al. A novel cyclic RGD-containing peptide polymer improves serum-free adhesion of adipose tissue-derived mesenchymal stem cells to bone implant surfaces. J Mater Sci: Mater Med 24, 479–488 (2013). https://doi.org/10.1007/s10856-012-4809-x

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  • DOI: https://doi.org/10.1007/s10856-012-4809-x

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