Abstract
The hypothesis for this study was that there is no difference in mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation between calcium-phosphate (CaP) coatings with different crystal size deposited on different topographic surfaces of metal discs. Polished (P) and sand-blasted (SB) tantalum and TiAl6V4 discs were CaP coated by three methods—biomimetic (BioM), electrochemical at 20 mA/cm2 and at 6.5 mA/cm2—and cultured with MSCs. At days 4, 7 and 14, cell proliferation—alamarBlue® activity and DNA quantification—and differentiation down the osteogenic lineage—ALP activity normalised per amount of DNA and SEM (morphology)—were analysed. Results showed that MSCs proliferated more when cultured on the nano-sized BioM coatings compared to uncoated and electrochemically coated discs. MSCs also proliferated more on P surfaces than on SB and or electrochemical coatings. All the coatings induced osteogenic differentiation, which was greater on electrochemical coatings and SB discs.
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García-Gareta, E., Hua, J., Knowles, J.C. et al. Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces. J Mater Sci: Mater Med 24, 199–210 (2013). https://doi.org/10.1007/s10856-012-4789-x
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DOI: https://doi.org/10.1007/s10856-012-4789-x