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Fabrication of biocompatible titanium scaffolds using space holder technique

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Abstract

Open-pore titanium scaffolds were fabricated by sintering of compressed mixtures of TiH1.924 and urea. Spherical and irregular shaped space holders were used to investigate the effect of pore shape on cellular behavior. After removal of the space holder, the shape of the spacers was replicated to the pores. Average diameter of the pores was in the range of 300–600 μm. SEM images showed that titanium hydride resulted in higher surface roughness and larger micro porosities than pure titanium. In vitro evaluations were carried out by using MTT assay, measuring alkaline phosphatase activity and alizarin red staining in flow perfusion bioreactor for cell culture. Observations revealed excellent attachment and proliferation of G-292 cells to the highly porous scaffolds fabricated with titanium hydride and urea of this research.

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References

  1. Hutmacher DW. Polymeric scaffolds in tissue engineering bone and cartilage. Biomaterials. 2000;21:2529–43.

    Article  CAS  Google Scholar 

  2. Freyman TM, Yannas IV, Gibson LJ. Cellular materials as porous scaffolds for tissue engineering. Prog Mater Sci. 2001;46:273–82.

    Article  CAS  Google Scholar 

  3. Jin QM, Takita H, Kohgo T, Atsumi K, Itoh H, Kuboki Y. Effects of geometry of hydroxyapatite as a cell substratum in BMP-induced ectopic bone formation. J Biomed Mater Res. 2000;51:491–9.

    Article  CAS  Google Scholar 

  4. Dong J, Kojima H, Uemura T, Kikuchi M, Tateishi T, Tanaka J. In vivo evaluation of a novel porous hydroxyapatite to sustain osteogenesis of transplanted bone marrow-derived osteoblastic cells. J Biomed Mater Res A. 2001;57:208–16.

    Article  CAS  Google Scholar 

  5. Damien E, Hing K, Saeed S, Revell PA. A preliminary study on the enhancement of the osteointegration of a novel synthetic hydroxyapatite scaffold in vivo. J Biomed Mater Res A. 2003;66:241–6.

    Article  Google Scholar 

  6. Takemoto M, Fujibayashi S, Neo M, Suzuki J, Kokubo T, Nakamura T. Mechanical properties and osteoconductivity of porous bioactive titanium. Biomaterials. 2005;26(6014–23):6014–23.

    Article  CAS  Google Scholar 

  7. Fujibayashi S, Neo M, Kim HM, Kokubo T, Nakamura T. Osteoinduction of porous bioactive titanium metal. Biomaterials. 2004;25:443–50.

    Article  CAS  Google Scholar 

  8. Ryan G, Pandit A, Apatsidis DP. Fabrication methods of porous metals for use in orthopaedic applications. Biomaterials. 2006;27:2651–70.

    Article  CAS  Google Scholar 

  9. Turner TM, Sumner DR, Urban RM, Rivero DP, Galante JO. A comparative study of porous coatings in a weight-bearing total hiparthroplasty model. J Bone Joint Surg. 1986;68:1396–409.

    CAS  Google Scholar 

  10. Cook SD, Walsh KA, Haddad RJ Jr. Interface mechanics and bone growth into porous Co–Cr–Mo alloy implants. Clin Orthop. 1985;193:271–80.

    CAS  Google Scholar 

  11. Clemow AJ, Weinstein AM, Klawitter JJ, Koeneman J, Anderson J. Interface mechanics of porous titanium implants. J Biomed Mater Res A. 1981;15:73–82.

    Article  CAS  Google Scholar 

  12. Cameron HU, Pilliar RM, Macnab I. The rate of bone ingrowth into porous metal. J Biomed Mater Res B. 1976;10:295–302.

    Article  CAS  Google Scholar 

  13. Mishra S, Knothe-Tate ML. Effect of lacunocanalicular architecture on hydraulic conductance in bone tissue: Implications for bone health and evolution. Anatomical Rec. 2003;273A:752–62.

    Article  Google Scholar 

  14. Bobyn J, Miller J. Features of biologically fixed devices. In: Simon, S.R. (Ed.) orthopaedic basic science. Am Acad Orthop Surg. 1994;10:613–6.

    Google Scholar 

  15. Bobyn J, Pilliar RM, Cameron H, Weatherly G. The optimum pore size for the fixation of porous surfaced metal implants by ingrowth of bone. Clin Orthop. 1980;150:263–70.

    Google Scholar 

  16. Itala AI, Ylanen HO, Ekholm C, Karlsson KH, Aro HT. Pore diameter of more than 100 micron is not requisite for bone ingrowth in rabbits. J Biomed Mater Res B. 2001;58:679–83.

    Article  CAS  Google Scholar 

  17. Wenjuan N, Chenguang B, GuiBao Q, Qiang W. Processing and properties of porous titanium using space holder technique. Mater Sci Eng A. 2009;506:148–51.

    Article  Google Scholar 

  18. Ye B, Dunand DC. Titanium foams produced by solid-state replication of NaCl powders. Mater Sci Eng A. 2010;528:691–7.

    Article  Google Scholar 

  19. Wen CE, Mabuchi M, Yamada Y, Shimojima K, Chino Y, Ashina T. Processing of biocompatible porous Ti and Mg. Scripta Mater. 2001;45:1147–53.

    Article  CAS  Google Scholar 

  20. Esen Z, Bor S. Processing of titanium foams using magnesium spacer particles. Scripta Mater. 2007;56:341–4.

    Article  CAS  Google Scholar 

  21. Ueno T, Tsukimura N, Yamada M, Ogawa T. Enhanced bone- integration capability of alkali- and heat-treated nanopolymorphic titanium in micro-to-nanoscale hierarchy. Biomaterials. 2011;32:7297–308.

    Article  CAS  Google Scholar 

  22. Fu Q, Hong Y, Liu X, Fan H, Zhang X. A hierarchically graded bioactive scaffold bonded to titanium substrates for attachment to bone. Biomaterials. 2011;32:7333–46.

    Article  CAS  Google Scholar 

  23. JCPDS. Powder diffraction file, ALPhabetical index. Inorganic Materials. Swarthmore: JCPDS; 1979.

    Google Scholar 

  24. Wu S, Liu X, Yeung KWK, Hu T, Xu Z, Chung JCY, Chu PK. Hydrogen release from titanium hydride in foaming of orthopedic NiTi scaffolds. Acta Biomater. 2011;7:1387–97.

    Article  CAS  Google Scholar 

  25. Sandim HRZ, Morante BV, Suzuki PA. Kinetics of thermal decomposition of titanium hydride powder using in situ high-temperature X-ray diffraction (HTXRD). Mater Res. 2005;8:293–7.

    Article  CAS  Google Scholar 

  26. Sugita Y, Ishizaki K, Iwasa F, Ueno T, Minamikawa H, Yamada M, Suzuki T, Ogawa T. Effects of pico-to-nanometer-thin TiO2 coating on the biological properties of microroughened titanium. Biomaterials. 2011;32:8374–84.

    Article  CAS  Google Scholar 

  27. Li JP, Habibovic P, Doel MV, Wilson CE, Wijn JRD, Blitterswijk CAV, Groot KD. Bone ingrowth in porous titanium implants produced by 3D fiber deposition. Biomaterials. 2007;28:2810–20.

    Article  CAS  Google Scholar 

  28. Zhang Q, Leng Y, Xin R. A comparative study of electrochemical deposition and biomimetic deposition of calcium phosphate on porous titanium. Biomaterials. 2005;26:2857–65.

    Article  CAS  Google Scholar 

  29. Wu Y, Zitelli JP, TenHuisen KS, Yu X, Libera MR. Differential response of staphylococci and osteoblasts to varying titanium surface roughness. Biomaterials. 2011;32:951–60.

    Article  Google Scholar 

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Acknowledgments

Kind cooperation of Mr. D. Shamaee who provided tools and instruments for fabrication of the scaffolds is greatly appreciated. Deputy of research of Sharif University of Technology is also thanked for continued support of Seed of Design and Accomplishment of New Processes for Production and Application of Advanced Materials.

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Authors and Affiliations

  1. Department of Materials Science and Engineering, Sharif University of Technology, PO Box 11365-9466, Tehran, Iran

    S. Naddaf Dezfuli & S. K. Sadrnezhaad

  2. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran

    S. Naddaf Dezfuli, M. A. Shokrgozar & S. Bonakdar

Authors
  1. S. Naddaf Dezfuli
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  2. S. K. Sadrnezhaad
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  3. M. A. Shokrgozar
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  4. S. Bonakdar
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Correspondence to S. K. Sadrnezhaad.

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Dezfuli, S.N., Sadrnezhaad, S.K., Shokrgozar, M.A. et al. Fabrication of biocompatible titanium scaffolds using space holder technique. J Mater Sci: Mater Med 23, 2483–2488 (2012). https://doi.org/10.1007/s10856-012-4706-3

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  • DOI: https://doi.org/10.1007/s10856-012-4706-3

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