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The healing of critical-size calvarial bone defects in rat with rhPDGF-BB, BMSCs, and β-TCP scaffolds

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Abstract

Platelet-derived growth factor-BB (PDGF-BB) plays important roles in regenerating damaged tissue. In this study we investigated the effects of a tissue-engineered bone combined with recombinant human PDGF-BB (rhPDGF-BB), bone marrow stem cells (BMSCs) and β–tricalcium phosphate (β-TCP) to repair critical-size calvarial bone defects in rat. Proliferation and osteogenic differentiation of BMSCs treated with different concentration rhPDGF-BB (0, 10, and 50 ng/ml) was evaluated by MTT, alkaline phosphatase (ALP) activity, alizarin red staining and real-time quantitative PCR (RT-qPCR) analysis of osteogenic gene. BMSCs were then combined with rhPDGF-BB-loaded β-TCP and transplanted into 5 mm calvarial bone defects. The new bone formation and mineralization was evaluated by micro-computerized tomography (Micro-CT) and histological analysis at week 8 after operation. It was observed that the proliferation of BMSCs treated with rhPDGF-BB was enhanced with a time- and dose- dependent manner. There were increased ALP activity, mineralized deposition and elevated mRNA levels of osteogenic gene for BMSCs treated with rhPDGF-BB, particularly in the 50 ng/ml group. Histological analysis showed new bone formation and mineralization in the rhPDGF-BB/BMSCs/β-TCP group was significantly higher than BMSCs/β-TCP, rhPDGF-BB/β-TCP, and β-TCP alone group (P < 0.05). In conclusion, rhPDGF-BB/BMSCs/β-TCP is a promising tissue-engineered bone for craniofacial bone regeneration.

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Acknowledgments

This work was supported by: National Natural Science Foundation of China 30772431, 30772434, 30973342; Program for New Century Excellent Talents in University NCET-08-0353, Science and Technology Commission of Shanghai Municipality 0952nm04000, 10430710900, 10dz2211600; Shanghai Education Committee T0203, 07SG19.

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Authors and Affiliations

  1. Department of Prosthodontics, School of Stomatology, Ninth People’s Hospital, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Research Institute of Stomatology, Shanghai, 200011, China

    Ling Xu, Kaige Lv, Wenjie Zhang & Fuqiang Zhang

  2. Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People’s Hospital, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China

    Xiuli Zhang & Xinquan Jiang

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  1. Ling Xu
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  2. Kaige Lv
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  3. Wenjie Zhang
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  4. Xiuli Zhang
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Correspondence to Xinquan Jiang or Fuqiang Zhang.

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Xu, L., Lv, K., Zhang, W. et al. The healing of critical-size calvarial bone defects in rat with rhPDGF-BB, BMSCs, and β-TCP scaffolds. J Mater Sci: Mater Med 23, 1073–1084 (2012). https://doi.org/10.1007/s10856-012-4558-x

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