Article

Journal of Materials Science: Materials in Medicine

, Volume 22, Issue 6, pp 1555-1567

Fabrication of nano-structured electrospun collagen scaffold intended for nerve tissue engineering

  • A. TimnakAffiliated withFaculty of Biomedical Engineering, Amirkabir University of Technology
  • , F. Yousefi GharebaghiAffiliated withFaculty of Biomedical Engineering, Amirkabir University of Technology
  • , R. Pajoum ShariatiAffiliated withFaculty of Chemical Engineering, Amirkabir University of Technology
  • , S. H. BahramiAffiliated withFaculty of Textile Engineering, Amirkabir University of Technology
  • , S. JavadianAffiliated withDepartment of Biochemistry, Pasteur Institute of Iran
  • , Sh. Hojjati EmamiAffiliated withFaculty of Biomedical Engineering, Amirkabir University of Technology
  • , M. A. ShokrgozarAffiliated withNational Cell Bank of Iran, Pasteur Institute of Iran Email author 

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Abstract

Nerve tissue engineering is one of the most promising methods in nerve tissue regeneration. The development of blended collagen and glycosaminoglycan scaffolds can potentially be used in many soft tissue engineering applications. In this study an attempt was made to develop two types of random and aligned electrospun, nanofibrous scaffold using collagen and a common type of glycosaminoglycan. Ion chromatography test, MTT and attachment assays were conducted respectively to trace the release of glycosaminoglycan, and to investigate the biocompatibility of the scaffold. Cell cultural tests showed that the scaffold acted as a positive factor to support connective tissue cell outgrowth. The positive effect of fiber orientation on cell outgrowth organization was traced through SEM images. Porosity percentage calculation and tensile strength measurement of the webs specified analogous properties to the native neural matrix tissue. These results suggested that nanostructured porous collagen-glycosaminoglycan scaffold is a potential cell carrier in nerve tissue engineering.