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Immobilization of RGD peptide on HA coating through a chemical bonding approach

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Abstract

In this work, Arg-Gly-Asp (RGD) sequence containing peptide was immobilized on hydroxyapatite (HA) coatings through a chemical bonding approach in two steps, surface modification with 3-aminopropyltriethoxysilane (APTES) and RGD immobilization. The results indicate that RGD has been successfully immobilized on HA coatings. Comparing with physical adsorption coatings, the chemically bonded RGD on the coatings shows much better anti-wash-out ability. Since RGD is able to recognize cell-membrane integrins on biointerfaces, the present method will be an effective way to favor interaction of cells with HA coatings.

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Acknowledgements

This work is partly supported by Heilongjiang Provincial Science Foundation (Grant for Young Scholars, No. QCO7C38).

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Authors and Affiliations

  1. Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou, Zhejiang, 310027, People’s Republic of China

    Chunli Yang, Kui Cheng & Wenjian Weng

  2. Depertment of Food Engineering, Harbin University of Commerce, Harbin, Heilongjiang, 150076, People’s Republic of China

    Chunli Yang & Chunyu Yang

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  1. Chunli Yang
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  2. Kui Cheng
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  3. Wenjian Weng
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  4. Chunyu Yang
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Correspondence to Wenjian Weng.

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Yang, C., Cheng, K., Weng, W. et al. Immobilization of RGD peptide on HA coating through a chemical bonding approach. J Mater Sci: Mater Med 20, 2349–2352 (2009). https://doi.org/10.1007/s10856-009-3794-1

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  • DOI: https://doi.org/10.1007/s10856-009-3794-1

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