Article

Journal of Materials Science: Materials in Medicine

, Volume 20, Issue 1, pp 177-183

Investigation of carbon nanofibers as support for bioactive substances

  • Liliana OlenicAffiliated withNational Institute for R&D of Isotopic and Molecular Technologies Email author 
  • , Gheorghe MihailescuAffiliated withNational Institute for R&D of Isotopic and Molecular Technologies
  • , Stela PruneanuAffiliated withNational Institute for R&D of Isotopic and Molecular TechnologiesSchool of Natural Sciences, Newcastle University
  • , Dan LupuAffiliated withNational Institute for R&D of Isotopic and Molecular Technologies
  • , Alexandru R. BirisAffiliated withNational Institute for R&D of Isotopic and Molecular Technologies
  • , Petre MargineanuAffiliated withNational Institute for R&D of Isotopic and Molecular Technologies
  • , Sorina GarabagiuAffiliated withNational Institute for R&D of Isotopic and Molecular Technologies
  • , Alexandru S. BirisAffiliated withNanotechnology Center and Applied Science Department, University of Arkansas at Little Rock

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Abstract

In this paper we have studied the adsorption properties of various bio-active systems onto the surface of carbon nanofibers (CNF) synthesized by chemical vapor deposition (CVD). Amino acids (alanine, aspartic acid, glutamic acid) and glucose oxidase (GOx) were adsorbed on CNF and the results were compared with those obtained when activated carbon (AC) was used as support. CNF and AC properties (hydrophilic or hydrophobic properties) were characterized by the pH value, the concentration of acidic/basic sites and by naphthalene adsorption. CNF with immobilized GOx was additionally investigated as a highly sensitive glucose biosensor. An amperometric method was used in an original manner to detect the changes in the specific activity of GOx, immobilized longer time on CNF. The method demonstrates that not the whole enzyme adsorbed onto CNF can catalyze the oxidation of glucose from the solution.