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Role of surface finishing on the in vitro biological properties of a silicon nitride–titanium nitride (Si3N4–TiN) composite

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Abstract

Silicon nitride (Si3N4) has been introduced clinically as an orthopedic biomaterial for interbody fusion devices and in joint replacements. However, the production of complex shapes through conventional mechanical machining is difficult and expensive and limits interesting applications. Thus, several electrically conductive reinforcements to the Si3N4 matrix, like TiN, have been proposed, generating composites suitable to be wrought by electrical discharge machining (EDM). In this study, Si3N4–TiN with high strength, low density, and good electric conductivity wrought by EDM was studied. The role of surface finishing was investigated comparing the interface generated during the EDM process to that resulting from further polishing. The different topographical features were assessed by electron microscopy, energy dispersive X-ray spectrometry, and profilometry. Surface wettability was also determined based on the measurement of the OCA of water and diiodomethane. The biological responses induced in MC3T3 cells, a widely diffused osteoblast model, were correlated with the surface pattern. The unpolished samples could promote better cell viability, with a more relevant effect on the cytoskeleton arrangement as highlighted by numerous cytoplasmic extensions and filopodia-like structures and the high number of focal adhesions, while MC3T3 cells grown on polished Si3N4–TiN specimens displayed a flat morphology. In addition, the unpolished Si3N4–TiN increased osteocalcin production and calcium deposition. Taken together, these data support the biocompatibility and in vitro osteogenic properties of the electroconductive Si3N4–TiN investigated. Further in vivo studies are required to explore the possible use of bone implants directly obtained by EDM.

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References

  1. Riley FL (2004) Silicon nitride and related materials. J Am Ceram Soc 83:245–265

    Article  Google Scholar 

  2. Guicciardi S, Melandri C, Medri V et al (2003) Effects of testing temperature and thermal treatments on some mechanical properties of a Si3N4–TiN composite. Mater Sci Eng A 360:35–45

    Article  Google Scholar 

  3. Bracisiewicz M, Medri V, Bellosi A (2002) Factors inducing degradation of properties after long term oxidation of Si3N4–TiN electroconductive composites. Appl Surf Sci 202:139–149

    Article  Google Scholar 

  4. Medri V, Bracisiewicz M, Krnel K et al (2005) Degradation of mechanical and electrical properties after long-term oxidation and corrosion of non-oxide structural ceramic composites. J Eur Ceram Soc 25:1723–1731

    Article  Google Scholar 

  5. McEntire BJ, Bal BS, Rahaman MN et al (2015) Ceramics and ceramic coatings in orthopaedics. J Eur Ceram Soc 35:4327–4369

    Article  Google Scholar 

  6. Kersten RF, van Gaalen SM, Arts MP et al (2014) The SNAP trial: a double blind multi-center randomized controlled trial of a silicon nitride versus a PEEK cage in transforaminal lumbar interbody fusion in patients with symptomatic degenerative lumbar disc disorders: study protocol. BMC Musculoskelet Disord 15:57

    Article  Google Scholar 

  7. Arts MP, Wolfs JFC, Corbin TP (2013) The CASCADE trial: effectiveness of ceramic versus PEEK cages for anterior cervical discectomy with interbody fusion; protocol of a blinded randomized controlled trial. BMC Musculoskelet Disord 14:244

    Article  Google Scholar 

  8. Zhou Y (2001) Study on the friction properties of silicon nitride-silicon nitride as a material for hip prostheses. Chin J Biomed Eng 20:326–329 + 334

  9. Bal BS, Khandkar A, Lakshminarayanan R et al (2009) Fabrication and testing of silicon nitride bearings in total hip arthroplasty. Winner of the 2007 ‘HAP’ PAUL Award. J Arthroplasty 24:110–116

    Article  Google Scholar 

  10. Bal BS, Rahaman MN (2012) Orthopedic applications of silicon nitride ceramics. Acta Biomater 8:2889–2898

    Article  Google Scholar 

  11. Roebben G, Sarbu C, Lube T et al (2004) Quantitative determination of the volume fraction of intergranular amorphous phase in sintered silicon nitride. Mater Sci Eng A 370:453–458

    Article  Google Scholar 

  12. Becher PF, Sun EY, Plucknett KP et al (1998) Microstructural design of silicon nitride with improved fracture toughness: I, effects of grain shape and size. J Am Ceram Soc 81:2821–2830

    Article  Google Scholar 

  13. Sun EY, Becher PF, Plucknett KP et al (1998) Microstructural design of silicon nitride with improved fracture toughness: II, effects of yttria and alumina additives. J Am Ceram Soc 81:2831–2840

    Article  Google Scholar 

  14. Becher PF (1991) Microstructural design of toughened ceramics. J Am Ceram Soc 74:1551–2916

    Article  Google Scholar 

  15. Neumann A, Reske T, Held M et al (2004) Comparative investigation of the biocompatibility of various silicon nitride ceramic qualities in vitro. J Mater Sci Mater Med 15:1135–1140

    Article  Google Scholar 

  16. Guedes e Silva CC, Higa OZ, Bressiani JC (2004) Cytotoxic evaluation of silicon nitride-based ceramics. Mater Sci Eng C 24:643–646

    Article  Google Scholar 

  17. Mazzocchi M, Bellosi A (2008) On the possibility of silicon nitride as a ceramic for structural orthopaedic implants. Part I: processing, microstructure, mechanical properties, cytotoxicity. J Mater Sci Mater Med 19:2881–2887

    Article  Google Scholar 

  18. Mazzocchi M, Gardini D, Traverso PL et al (2008) On the possibility of silicon nitride as a ceramic for structural orthopaedic implants. Part II: chemical stability and wear resistance in body environment. J Mater Sci Mater Med 19:2889–2901

    Article  Google Scholar 

  19. Santos C, Ribeiro S, Daguano JKMF et al (2007) Development and cytotoxicity evaluation of SiAlONs ceramics. Mater Sci Eng C 27:148–153

    Article  Google Scholar 

  20. Cappi B, Neuss S, Salber J et al (2010) Cytocompatibility of high strength non-oxide ceramics. J Biomed Mater Res Part A 93:67–76

    Google Scholar 

  21. Howlett CR, McCartney E, Ching W (1989) The effect of silicon nitride ceramic on rabbit skeletal cells and tissue. Clin Orthop Relat Res 244:293–304

    Google Scholar 

  22. Neumann A, Kramps M, Ragoß C et al (2004) Histological and microradiographic appearances of silicon nitride and aluminum oxide in a rabbit femur implantation model. Materwiss Werksttech 35:569–573

    Article  Google Scholar 

  23. Neumann A, Unkel C, Werry C et al (2006) Prototype of a silicon nitride ceramic-based miniplate osteofixation system for the midface. Otolaryngol Head Neck Surg 134:923–930

    Article  Google Scholar 

  24. Rak K, Wasielewski N, Radeloff A et al (2011) Growth behavior of cochlear nucleus neuronal cells on semiconductor substrates. J Biomed Mater Res Part A 97A:158–166

    Article  Google Scholar 

  25. Carter EA, Rayner BS, McLeod AI et al (2010) Silicon nitride as a versatile growth substrate for microspectroscopic imaging and mapping of individual cells. Mol BioSyst 6:1316–1322

    Article  Google Scholar 

  26. Hirata I, Iwata H, Ismail ABM et al (2000) Surface modification of Si3N4-coated silicon plate for investigation of living cells. Jpn J Appl Phys Part 1 Regul Pap Short Notes Rev Pap 39:6441–6442

  27. Guedes E, Silva CC, König B, Carbonari MJ et al (2008) Tissue response around silicon nitride implants in rabbits. J Biomed Mater Res Part A 84:337–343

    Article  Google Scholar 

  28. Guedes e Silva CC, König B, Carbonari MJ et al (2008) Bone growth around silicon nitride implants—an evaluation by scanning electron microscopy. Mater Charact 59:1339–1341

    Article  Google Scholar 

  29. Anderson MC, Olsen R (2010) Bone ingrowth into porous silicon nitride. J Biomed Mater Res Part A 92:1598–1605

    Google Scholar 

  30. Webster TJ, Patel AA, Rahaman MN et al (2012) Anti-infective and osteointegration properties of silicon nitride, poly(ether ether ketone), and titanium implants. Acta Biomater 8:4447–4454

    Article  Google Scholar 

  31. Gorth DJ, Puckett S, Ercan B et al (2012) Decreased bacteria activity on Si3N4 surfaces compared with PEEK or titanium. Int J Nanomed 7:4829–4840

    Google Scholar 

  32. Herrmann M, Balzer B, Schubert C et al (1993) Densification, microstructure and properties of Si3N4Ti(C, N) composites. J Eur Ceram Soc 12:287–296

    Article  Google Scholar 

  33. Bellosi A, Guicciardi S, Tampieri A (1992) Development and characterization of electroconductive Si3N4–TiN composites. J Eur Ceram Soc 9:83–93

    Article  Google Scholar 

  34. Bucciotti F, Mazzocchi M, Bellosi A (2010) Perspectives of the Si3N4–TiN ceramic composite as a biomaterial and manufacturing of complex-shaped implantable devices by electrical discharge machining (EDM). J Appl Biomater Biomech 8:28–32

    Google Scholar 

  35. Martin C, Cales B, Vivier P et al (1989) Electrical discharge machinable ceramic composites. Mater Sci Eng A 109:351–356

    Article  Google Scholar 

  36. Canullo L, Genova T, Tallarico M et al (2016) Plasma of argon affects the earliest biological response of different implant surfaces: an in vitro comparative study. J Dent Res 95:566–573

    Article  Google Scholar 

  37. Ström G, Fredriksson M, Stenius P (1987) Contact angles, work of adhesion, and interfacial tensions at a dissolving hydrocarbon surface. J Colloid Interface Sci 119:352–361

    Article  Google Scholar 

  38. Gould RF, Gould Robert F. Contact angle, wettability, and adhesion. Washington, D.C.: American Chemical Society. doi:10.1021/ba-1964-0043. (Epub ahead of print 1 January 1964)

  39. Owens DK, Wendt RC (1969) Estimation of the surface free energy of polymers. J Appl Polym Sci 13:1741–1747

    Article  Google Scholar 

  40. Mussano F, Lee KJ, Zuk P et al (2010) Differential effect of ionizing radiation exposure on multipotent and differentiation-restricted bone marrow mesenchymal stem cells. J Cell Biochem 111:322–332

    Article  Google Scholar 

  41. Passeri G, Cacchioli A, Ravanetti F et al (2010) Adhesion pattern and growth of primary human osteoblastic cells on five commercially available titanium surfaces. Clin Oral Implants Res 21:756–765

    Article  Google Scholar 

  42. Albrektsson T, Wennerberg A (2004) Oral implant surfaces: part 1—review focusing on topographic and chemical properties of different surfaces and in vivo responses to them. Int J Prosthodont 17:536–543

    Google Scholar 

  43. Bock RM, McEntire BJ, Bal BS et al (2015) Surface modulation of silicon nitride ceramics for orthopaedic applications. Acta Biomater 26:318–330

    Article  Google Scholar 

  44. Gristina R, D’Aloia E, Senesi GS et al (2009) Increasing cell adhesion on plasma deposited fluorocarbon coatings by changing the surface topography. J Biomed Mater Res B Appl Biomater 88:139–149

    Article  Google Scholar 

  45. Genova T, Munaron L, Carossa S et al (2016) Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’. J Biomater Appl 30:940–951

    Article  Google Scholar 

  46. Martin JY, Schwartz Z, Hummert TW et al (1995) Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). J Biomed Mater Res 29:389–401

    Article  Google Scholar 

  47. Geiger B, Bershadsky A, Pankov R et al (2001) Transmembrane extracellular matrix—cytoskeleton crosstalk. Nat Rev Mol Cell Biol 2:793–805

    Article  Google Scholar 

  48. Anselme K (2000) Osteoblast adhesion on biomaterials. Biomaterials 21:667–681

    Article  Google Scholar 

  49. Qu Z, Rausch-Fan X, Wieland M et al (2007) The initial attachment and subsequent behavior regulation of osteoblasts by dental implant surface modification. J Biomed Mater Res A 82:658–668

    Article  Google Scholar 

  50. Zhao G, Raines AL, Wieland M et al (2007) Requirement for both micron- and submicron scale structure for synergistic responses of osteoblasts to substrate surface energy and topography. Biomaterials 28:2821–2829

    Article  Google Scholar 

Download references

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Author notes

    Authors and Affiliations

    1. Department of Surgical Sciences UNITO, CIR Dental School, via Nizza 230, 10126, Turin, Italy

      F. Mussano, T. Genova & S. Carossa

    2. Department of Life Sciences and Systems Biology, UNITO, via Accademia Albertina 13, 10123, Turin, Italy

      T. Genova & L. Munaron

    3. Department of Applied Science and Technology, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129, Turin, Italy

      P. Rivolo & P. Mandracci

    4. Centre for Nanostructured Interfaces and Surfaces (NIS), Turin, Italy

      L. Munaron

    5. IMAMOTER-National Council of Research, Strada delle Cacce 73, 10135, Turin, Italy

      M. G. Faga

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    1. F. Mussano
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    2. T. Genova
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    Correspondence to F. Mussano.

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    F. Mussano and T. Genova contributed equally to the paper.

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    Mussano, F., Genova, T., Rivolo, P. et al. Role of surface finishing on the in vitro biological properties of a silicon nitride–titanium nitride (Si3N4–TiN) composite. J Mater Sci 52, 467–477 (2017). https://doi.org/10.1007/s10853-016-0346-1

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    • DOI: https://doi.org/10.1007/s10853-016-0346-1

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