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Electrochemical template synthesis of protein-imprinted magnetic polymer microrods

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Abstract

A novel method for the electrochemical template synthesis of surface-imprinted magnetic polymer microrods for protein recognition is proposed. The polymer was electrodeposited into sacrificial cylindrical microreactors, the internal walls of which were previously modified with a target model protein, avidin, by simple physisorption. The electropolymerization was performed from a mixture of 3,4-ethylenedioxythiophene, poly(styrenesulfonate) (PSS), and PSS-coated superparamagnetic nanoparticles resulting in the formation of inherently electroconductive polymers confined to the volume of the microreactor. Here we show that: (i) the template synthesis within cylindrical microreactors results in polymer rods with dimensions matching that of the reactor, (ii) the incorporation of superparamagnetic particles induces magnetic properties that allow for efficient collection and manipulation of the microrods released from the microreactors in magnetic field even from dilute solution, and (iii) the protein coating on the internal walls of the microreactors is shown to generate molecular imprints on the surface of the polymeric rods. This latter property was demonstrated by comparative binding experiments of a fluorescent avidin derivative to the surface-imprinted and non-imprinted magnetic polymer microrods.

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Acknowledgements

This work is connected to the scientific program of the “Development of quality-oriented and harmonized R + D + I strategy and functional model at BME” project (TÁMOP-4.2.1/B-09/1/KMR-2010–0002). This work was supported by the European Commission (MRTN-CT-2006-033873) and Hungarian OTKA under Grant Nos. PD75615, K104724, and CNK80991. The authors would like to thank Dr. László Ferenc Kiss for the assistance during the magnetization measurements and Prof. László Bezúr for the atomic absorption measurements.

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Authors and Affiliations

  1. Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4., Budapest, 1111, Hungary

    Giorgio Ceolin & Róbert E. Gyurcsányi

  2. Department of Physics, Budapest University of Technology and Economics and Condensed Matter Research Group of the Hungarian Academy of Sciences, Budafoki út 8., Budapest, 1111, Hungary

    Ágnes Orbán, Vilmos Kocsis & István Kézsmárki

  3. MTA-BME Research Group of Technical Analytical Chemistry, Hungarian Academy of Sciences, Budapest University of Technology and Economics, Szt. Gellért tér 4., Budapest, 1111, Hungary

    Róbert E. Gyurcsányi & Viola Horváth

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  1. Giorgio Ceolin
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  2. Ágnes Orbán
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  3. Vilmos Kocsis
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  6. Viola Horváth
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Correspondence to Viola Horváth.

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Ceolin, G., Orbán, Á., Kocsis, V. et al. Electrochemical template synthesis of protein-imprinted magnetic polymer microrods. J Mater Sci 48, 5209–5218 (2013). https://doi.org/10.1007/s10853-013-7309-6

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  • DOI: https://doi.org/10.1007/s10853-013-7309-6

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