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Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection

  • Assisted Reproduction Technologies
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Abstract

Purpose

In intracytoplasmic sperm injection (ICSI) of oocytes with a fragile oolemma (fragile oocytes), breakage can occur at injection. In this study, we produced embryos from oocytes with a fragile and normal oolemma (normal oocytes) by ICSI and compared their ability to be fertilized and develop in vitro. We also investigated whether fragile oocyte-derived embryos could implant after blastocyst transfer to determine whether fragile oocytes should be used for assisted reproductive technology treatment.

Methods

Oocytes were divided into three groups—normal oocytes from cycles containing no fragile oocytes (group A), normal oocytes from cycles containing at least one fragile oocyte (group B), and fragile oocytes (group C), and their fertilization abilities after ICSI and the developmental abilities of resultant embryos were compared.

Results

The fertilization rate in group C (65.3 %) was significantly (P < 0.01) lower than those in groups A (84.6 %) and B (86.9 %), and the degeneration rate in group C (24.2 %) was significantly (P < 0.01) higher than those in groups A (0.71 %) and B (0.28 %). However, there were no significant differences in the blastocyst formation rates (59.7–67.5 %) of embryos among the different groups. In addition, the pregnancy rate after transfer of blastocysts in group C (50.0 %) was not significantly different from those in groups A (35.6 %) and B (45.8 %).

Conclusions

The fertilization ability after ICSI of fragile oocytes is lower than that of normal oocytes but the resultant embryos have the same developmental ability as those of normal oocyte-derived embryos.

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Acknowledgments

We would like to thank the staff of the Aiiku Ladies Clinic (Kagoshima, Japan).

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Authors and Affiliations

  1. Aiiku Ladies Clinic, 1-40-2 Komatsubara, Kagoshima, 891-0114, Japan

    Yamato Mizobe, Naoto Oya, Reiko Iwakiri, Naomi Yoshida, Yumi Sato, Nanase Onoue, Makoto Tokunaga & Yuji Ezono

  2. The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan

    Yamato Mizobe & Kazuchika Miyoshi

Authors
  1. Yamato Mizobe
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  2. Naoto Oya
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  4. Naomi Yoshida
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  6. Nanase Onoue
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Corresponding author

Correspondence to Yamato Mizobe.

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Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants

This study was approved by the Institutional Review Board of AIIKUKAI Medical Corporation.

Informed consent

Written informed consent for their treatment and for their outcomes to be described was obtained from all patients.

Additional information

Capsule In intracytoplasmic sperm injection (ICSI) of oocytes with fragile oolemma, breakage can occur at injection. We produced embryos by ICSI of oocytes with fragile and normal oolemma, compared fertilization and development in vitro, and implanted fragile oocyte-derived embryos after blastocyst transfer to determine usability of fragile oocytes in assisted reproduction.

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Mizobe, Y., Oya, N., Iwakiri, R. et al. Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection. J Assist Reprod Genet 33, 1685–1690 (2016). https://doi.org/10.1007/s10815-016-0811-4

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  • DOI: https://doi.org/10.1007/s10815-016-0811-4

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