Skip to main content
SpringerLink
Log in

Clinical efficiency of Piezo-ICSI using micropipettes with a wall thickness of 0.625 μm

  • Technological Innovations
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

An Erratum to this article was published on 27 February 2016

Abstract

Purpose

The purposes of the present study are to assess the clinical efficiency of Piezo-intracytoplasmic sperm injection (ICSI) and to improve the Piezo-ICSI method for human oocytes.

Methods

We examined three ICSI methods to determine their clinical efficiency by comparing the survival, fertilization, good-quality day-3 embryo, pregnancy, and live birth rates. The three ICSI methods tested were conventional ICSI (CI) (using beveled spiked micropipettes with a wall thickness of 1 μm), conventional Piezo-ICSI (CPI) (using flat-tipped micropipettes with a wall thickness of 0.925 μm), and improved Piezo-ICSI (IPI) (using flat-tipped micropipettes with a wall thickness of 0.625 μm). We collectively investigated 2020 mature oocytes retrieved from 437 patients between October 2010 and January 2014.

Results

The survival rates after CI, CPI, and IPI were 90, 95, and 99 %, respectively. The fertilization rates after CI, CPI, and IPI were 68, 75, and 89 %, respectively. The good-quality day-3 embryo rates after CI, CPI, and IPI were 37, 43, and 55 %, respectively. The pregnancy rates after the transfer of good-quality day-3 embryo of CI, CPI, and IPI were 19, 21, and 31 %, respectively. The live birth rates of CI, CPI, and IPI were 15, 16, and 25 %, respectively. Significantly higher survival, fertilization, good-quality day-3 embryo, pregnancy, and live birth rates were obtained using IPI.

Conclusions

When comparing the IPI to the CI and CPI, the results revealed that the Piezo-ICSI using flat-tipped micropipettes with a wall thickness of 0.625 μm significantly improves survival, fertilization, good-quality day-3 embryo, pregnancy, and live birth rates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;340:17–8.

    Article  CAS  PubMed  Google Scholar 

  2. Kimura Y, Yanagimachi R. Intracytoplasmic sperm injection in the mouse. Biol Reprod. 1995;52:709–20.

    Article  CAS  PubMed  Google Scholar 

  3. Huang T, Kimura Y, Yanagimachi R. The use of piezo micromanipulation for intracytoplasmic sperm injection of human oocytes. J Assist Reprod Genet. 1996;13:320–8.

    Article  CAS  PubMed  Google Scholar 

  4. Yanagida K, Katayose H, Yazawa H, Kimura Y, Konnai K, Sato A. The usefulness of a piezo-micromanipulator in intracytoplasmic sperm injection in humans. Hum Reprod. 1999;14:448–53.

    Article  CAS  PubMed  Google Scholar 

  5. Takeuchi S, Minoura H, Shibahara T, Shen X, Futamura N, Toyoda N. Comparison of piezo-assisted micromanipulation with conventional micromanipulation for intracytoplasmic sperm injection into human oocytes. Gynecol Obstet Investig. 2001;52:158–62.

    Article  CAS  Google Scholar 

  6. Ferraretti AP, Gianaroli L, Magli MC, Devroey P. Mild ovarian stimulation with clomiphene citrate launch is a realistic option for in vitro fertilization. Fertil Steril. 2015. doi:10.1016/j.fertnstert.2015.05.006.

    Google Scholar 

  7. Diedrich K, Diedrich C, Santos E, Zoll C, al-Hasani S, Reissmann T, et al. Suppression of the endogenous luteinizing hormone surge by the gonadotrophin-releasing hormone antagonist Cetrorelix during ovarian stimulation. Hum Reprod. 1994;9(5):788–91.

    CAS  PubMed  Google Scholar 

  8. Kuwayama M, Vajta G, Ieda S, Kato O. Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Reprod Biomed Online. 2005;11:608–14.

    Article  PubMed  Google Scholar 

  9. Hiraoka K, Hiraoka K, Horiuchi T, Kusuda T, Okano S, Kinutani M, et al. Impact of the size of zona pellucida thinning area on vitrified-warmed cleavage-stage embryo transfers: a prospective, randomized study. J Assist Reprod Genet. 2009;26:515–21.

    Article  PubMed Central  PubMed  Google Scholar 

  10. Dumoulin JM, Coonen E, Bras M, Bergers-Janssen JM, Ignoul-Vanvuchelen RC, van Wissen LC, et al. Embryo development and chromosomal anomalies after ICSI: effect of the injection procedure. Hum Reprod. 2001;16:306–12.

    Article  CAS  PubMed  Google Scholar 

  11. Abdelmassih S, Cardoso J, Abdelmassih V, Dias JA, Abdelmassih R, Nagy ZP. Laser-assisted ICSI: a novel approach to obtain higher oocyte survival and embryo quality rates. Hum Reprod. 2002;17:2694–9.

    Article  CAS  PubMed  Google Scholar 

  12. Ebner T, Moser M, Sommergruber M, Jesacher K, Tews G. Complete oocyte activation failure after ICSI can be overcome by a modified injection technique. Hum Reprod. 2004;19:1837–41.

    Article  CAS  PubMed  Google Scholar 

  13. Richter KS, Davis A, Carter J, Greenhouse SJ, Mottla GL, Tucker MJ. No advantage of laser-assisted over conventional intracytoplasmic sperm injection: a randomized controlled trial. J Exp Clin Assist Reprod. 2006;3:5.

    Article  PubMed Central  PubMed  Google Scholar 

  14. De Vos A, Van Landuyt L, Van Ranst H, Vandermonde A, D’Haese V, Sterckx J, et al. Randomized sibling-oocyte study using recombinant human hyaluronidase versus bovine-derived Sigma hyaluronidase in ICSI patients. Hum Reprod. 2008;23:1815–9.

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

We are indebted to Ms. Adair Oesterle for her critical reading of the manuscript.

Author information

Authors and Affiliations

  1. Kameda Medical Center, 929 Higashi-cho, Kamogawa-shi, Chiba, 296-8602, Japan

    Kenichiro Hiraoka

  2. Ogikubo Hospital Niji Clinic, 4-32-2-8F9F Ogikubo, Suginami-ku, Tokyo, 167-0051, Japan

    Seiji Kitamura

Authors
  1. Kenichiro Hiraoka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seiji Kitamura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kenichiro Hiraoka.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. For this type of study, formal consent is not required.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Capsule Piezo-ICSI, in combination with flat-tipped micropipettes with a wall thickness of 0.625 μm, significantly improves survival, fertilization, good-quality day-3 embryo, pregnancy, and live birth rates as compared to conventional ICSI.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hiraoka, K., Kitamura, S. Clinical efficiency of Piezo-ICSI using micropipettes with a wall thickness of 0.625 μm. J Assist Reprod Genet 32, 1827–1833 (2015). https://doi.org/10.1007/s10815-015-0597-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10815-015-0597-9

Keywords

Search

Navigation